- A proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes
- Greater than 90% of the clones are in the correct orientation for gene expression, reducing the time spent on colony screening
E. coli,Bacterial Expression Kits" />
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T7 and lacO
|Bacterial or Yeast Strain:||
EK (Enterokinase) Recognition Site
|Protein Tag or Fusion:||
Xpress Epitope Tag,
His Tag (6x)
|Selection Agent (Eukaryotic):||
|Constitutive or Inducible System:||
|Antibiotic Resistance (Bacterial):||
For Research Use Only. Not for use in diagnostic procedures.
Each Champion™ pET Directional TOPO® Expression Kit is provided as a complete expression system. The Directional TOPO® Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot® TOP10 box contains twenty-one 50-µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot® BL21 Star™(DE3) box contains twenty-one 50-µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 µl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Note: Use lot number only (e.g., "K18126").
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