Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
|Concentration||15 U/ µL|
|Contents||Xba I 10X Buffer M (1mL) 10X Loading Buffer (1mL) |
|Reaction Mixture ||Xba I 1 µL 10X Buffer M 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL |
|Heat inactivation||Enzyme inactivated by heating at 65°C for 20 minutes.|
|Star Activity||Unrelated site may be cut in the presence of high concentration of glycerol.|
|Effect of DNA Methylation||When the sequence includes the recognition site TCTAGATC, the enzyme activity is affected by dam methylase. In this case, DNA originated from E. coli cannot be cleaved by this enzyme.|
|Unit Definition||One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.|
|10X Buffer M|| 100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 500 mM NaCl |
Relative Activity in Universal Reaction Buffers
|Relative Activity (%)||<20||80*||20||<20||120|
*100% activity is obtained after addition of 0.01% BSA. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.