The 1 Kb Plus DNA ladder is our most popular DNA ladder used to estimate the molecular mass of double-stranded DNA bands from PCR, restriction digests, and other molecular biology protocols. Unlike some other DNA ladders, the 1 Kb Plus DNA ladder has been specially designed to have bands of round, easy-to-remember sizes. The logical band layout, and quick identification doublet, allow you to quickly and easily estimate the size of your band of interest at a glance (see figure).
A single ladder for most of your needs
The 20 bands comprising the 1 Kb Plus DNA Ladder range in size from 100 bp up to 12 Kb, making it one of the broadest DNA ladders available. This wide breadth is perfect for downstream cloning applications as well as end-point PCR and other molecular biology applications.
Simplified format for use with all DNA agarose gels
The 1 Kb Plus DNA ladder is compatible with all agarose gels that can be used to analyze DNA fragments, including self-cast agarose gels and E-Gel® products. The ladder is provided in a purified format without premixed loading buffer. The ladder should be mixed with an appropriate loading buffer prior to being loaded onto an agarose gel. The 1 Kb Plus DNA Ladder is supplied at 1 µg/µL in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 1 mM EDTA. Store at -20°C, and for best results aliquot stock to avoid repeated freeze-thaw cycles.
DNA stain and radiolabel compatible
To visualize the 1 Kb Plus DNA Ladder, stain with SYBR® Safe, ethidium bromide, or a similar DNA stain. The 1 Kb Plus DNA Ladder can also be radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment of DNA polymerase I (Klenow fragment). Fill-in labeling can use any radiolabeled dNTP.