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|Protein Tag or Fusion:||
|Constitutive or Inducible System:||
|Selection Agent (Eukaryotic):||
For Research Use Only. Not for use in diagnostic procedures.
Box 1 (store at -20°C)
• 20 µl pcDNA™3.3-TOPO® vector, TOPO® adapted (5–10 ng/µl)
• 100 µl 10X PCR Buffer
• 10 µl dNTP Mix
• 50 µl Salt Solution
• 1 ml Sterile Water
• 10 µl Control PCR Template (50 ng/µl)
• 10 µl Control PCR Primers (100 ng/µl each)
• 20 µl CMV Forward Sequencing Primer (100 ng/µl)
• 20 µl TK polyA Reverse Sequencing Primer (100 ng/µl)
• 10 µl pcDNA™3.3-TOPO®/lacZ Expression Control Plasmid (500 ng/µl)
Box 2 (store at -80°C)
• 11 × 50 µl TOP10 E. coli
• 50 µl pUC19 Control DNA (10 pg/µl)
• 6 ml S.O.C. Medium
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