This M199 is modified as follows:
|• Phenol Red||• HEPES|
The complete formulation is available.
Using Medium 199
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hank's salts for use without CO2. This product contains Earle's salts. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
Medium 199 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Medium 199 product made in our Scotland facility (31150-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.