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Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g., in the presence of SDS or urea) • In the presence of metal chelating agents (e.g., EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
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