The iBlot® Gel Transfer Device performs western blotting transfer efficiently and reliably, typically in seven minutes or less without the need for liquid buffers. The iBlot® Gel Transfer Device is an integral part of the iBlot® Dry Blotting System, which consists of the transfer device and disposable blotting stacks. which contain the buffers and the transfer membrane (nitrocellulose or PVDF).
Using the iBlot® Gel Transfer Device
The iBlot® Dry Blotting System is for dry, electroblotting of proteins from mini-, midi-, and E-PAGE™ gels, electroblotting of nucleic acids from agarose gels and TBE polyacrylamide gels, and performing western detection on nitrocellulose or PVDF membranes. The iBlot® system offers high-quality transfer, convenience, and speed. The device and the stacks are sold separately. The iBlot® system is ideal for scientists analyzing proteins through western blotting who want:
• Complete protein transfer in seven minutes or less
• High detection sensitivity and evenness
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
How it works
Buffer ion reservoirs are incorporated in the gel matrix (transfer stacks) instead of buffer tanks or soaked papers (see figure). The high density and availability of ions in the gel matrix enables rapid protein transfers. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, which can cause blotting distortion. Conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes which generate oxygen. With the iBlot® Dry Blotting System, transfer time is reduced by using a shortened distance between electrodes, high field strength, and high currents. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are avoided using a unique de-bubbling design to ensure even and complete transfers.
iBlot® transfers save you time
With the iBlot® system, there is no need to prepare buffers, pre-treat your gel, or clean up after your blotting. The total preparation and run time is normally less than 10 minutes per blot. Compared to conventional western transfer techniques, iBlot® transfers save you hours (see table).
Quality protein transfers
The iBlot® Dry Blotting System transfers proteins evenly and efficiently from gels. With iBlot® transfers, crisp, clear bands remain sharp and straight with high transfer efficiency (see figure).