Manufactured by Sino Biological (China); Distributed by Life Technologies.
Cadherin 1, type 1, E-cadherin (epithelial) (CDH1) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein is expressed from a DNA sequence encoding the extracellular domain of human CDH1 precusor (NP_004351.1) (Met 1-Ile 707) fused to the polyhistidine tag at the C-terminus.N-terminal Sequence Analysis:
Gln 23 & Asp 155.Activity:
measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells . When cells are added to CDH1/ECAD coated plates (1.5 µg/mL, 100 µL/well), approximately 60 %-80 % will adhere specifically .Formulation:
lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2
, 1.8 mM KH2
, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expiration Date:
expires one year from date of receipt when stored as instructed.Background
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin.You may also be interested in the following CDH1 products: Primary AntibodiesGene Expression Assays