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Ion PGM™ System
Targeted Sequencing Library
Compatible with Ion Xpress™ Barcode Kits
|Sample Type (Specific):||
|Starting Material (Amount):||
500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
For Research Use Only. Not for use in diagnostic procedures.
Ion AmpliSeq Library Kit 2.0
1 tube 5X Ion AmpliSeq HiFi Master Mix - Storage: -30 to -10 °C
1 tube FuPa Reagent - Storage: -30 to -10 °C
1 tube Switch Solution - Storage: -30 to -10 °C
1 tube DNA Ligase - Storage: -30 to -10 °C
1 tube Ion AmpliSeq Adapters - Storage: -30 to -10 °C
1 tube Platinum PCR SuperMix HiFi - Storage: -30 to -10 °C
1 tube Library Amplification Primer Kit - Storage: -30 to -10 °C
1 tube Low TE - Storage: Room Temperature
Ion AmpliSeq RNA RT Module
1 tube 5X VILO RT Reaction Mix - Storage: -25 to -15 °C
1 tube 10X SuperScript III Enzyme Mix - Storage: -25 to -15 °C
Ion AmpliSeq DynaBeads Cleanup Module
1 tube Binding Solution Concentrate - Storage: Room Temperature
1 tube Wash Solution Concentrate - Storage: Room Temperature
1 tube Dynabeads Magnetic Beads - Storage: 2 to 8 °C
2 tubes Nuclease Free Water - Storage: Room Temperature
1 Processing Plate & Lid - Storage: Room Temperature
Note: Use lot number only (e.g., "K18126").
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