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Ion PGM™ System
Targeted Sequencing Library
Compatible with Ion Xpress™ Barcode Kits
|Sample Type (Specific):||
|Starting Material (Amount):||
500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
For Research Use Only. Not for use in diagnostic procedures.
Ion AmpliSeq Library Kit 2.0
4 tubes 5X Ion AmpliSeq HiFi Master Mix - Storage: -30 to -10 °C
4 tubes FuPa Reagent - Storage: -30 to -10 °C
4 tubes Switch Solution - Storage: -30 to -10 °C
4 tubes DNA Ligase - Storage: -30 to -10 °C
4 tubes Ion AmpliSeq Adapters - Storage: -30 to -10 °C
12 tubes Platinum PCR SuperMix HiFi - Storage: -30 to -10 °C
4 tubes Library Amplification Primer Kit - Storage: -30 to -10 °C
4 tubes Low TE - Storage: Room Temperature
Ion AmpliSeq RNA RT Module
4 tubes 5X VILO RT Reaction Mix - Storage: -25 to -15 °C
4 tubes 10X SuperScript III Enzyme Mix - Storage: -25 to -15 °C
Ion AmpliSeq DynaBeads Cleanup Module
4 tubes Binding Solution Concentrate - Storage: Room Temperature
4 tubes Wash Solution Concentrate - Storage: Room Temperature
4 tubes Dynabeads Magnetic Beads - Storage: 2 to 8 °C
4 tubes Nuclease Free Water - Storage: Room Temperature
16 Processing Plate & Lid - Storage: Room Temperature
Note: Use lot number only (e.g., "K18126").
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