Create a perfectly labeled antibody with the SiteClick™ R-PE Antibody Labeling Kit. Unlike conventional antibody labeling kits, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Depending upon the label, the resulting SiteClick™ labeled antibody can be used in flow cytometry or any other application.
Important Features of the SiteClick™ R-PE Antibody Labeling Kit:
• Contains everything required to label 100 µg of IgG antibody
• Easy to follow step-by-step protocol
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugate. R-PE (R- Phycoerythrin) as a Bright Label
R-Phycoerythrin (R-PE) is a bright phycobilliprotein extracted from red algae. Its primary absorption (excitation) peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum and extremely bright fluorescence (emission maxima = ~578 nm) makes it a useful label for multiplexing in immunocytochemisty, especially in flow cytometry and cell sorting( FACS) applications. Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes® antibody and protein labeling kits (see Antibody Labeling from A to Z
). To learn more about our various kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2
in the Molecular Probes® Handbook. Custom SiteClick™ Antibody Labeling Services
If you have an antibody that is considered "difficult to label" or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick™ Antibody Labeling Service would be right for your antibody. Life Technologies offers complete custom SiteClick™ antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor® dyes, Qdot® fluorophores, R-PE, and others.