Manufactured by Sino Biological (China); Distributed by Life Technologies.
Low density lipoprotein receptor (LDLR) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
This recombinant human protein is expressed from a DNA sequence encoding the extracellular domain of human LDLR precursor (NP_000518.1), residues 1 to 788, fused to a polyhistidine tag at the C-terminus.N-terminal Sequence Analysis:
Measure by its ability to bind to human PCSK9 in a functional ELISA.
1. Immobilized human PCSK9 at 10 µg/mL(100 µL/well) has been shown to bind biotinylated recombinant human LDLR. The ED50
of biotinylated human LDLR is 0.61 µg/mL.
2. Immobilized mouse PCSK9 at 10 µg/mL(100 µL/well) has been shown to bind biotinylated recombinant human LDLR. The ED50
of biotinylated human LDLR is 0.12 µg/mL.Formulation:
lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expiration Date:
expires one year from date of receipt when stored as instructed.Background
Low-density lipoprotein (LDL) is one of the five major groups of lipoprotein that transports cholesterol and triglycerides from the liver to peripheral tissues, and enables fats and cholesterol to move within the water-based solution of the blood stream. The Low-Density Lipoprotein Receptor (LDLR) is the prototype member of the LDLR family of scavenger receptors which is involved in receptor-mediated endocytosis of specific ligands. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. LDLR recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles, as well as the apoE protein found in chylomicron remnants and VLDL remnants (IDL). After taken into the cell ending up in lysosomes, LDL is degraded and the cholesterol is made available for repression of microsomal enzyme HMG CoA reductase, the rate-limiting step in cholesterol synthesis. As a mosaic protein, the extracellular region of LDLR consists of seven Nterminal complement-like cysteinerich repeats followed by three EGF like repeats with a β-propeller YWTD containing motif, and a membrane proximal Ser/Thr rich region. Furthermore, it is reported that in case of HIV-1 infection, LDLR functions as a receptor for extracellular Tat (HIV-1 transactivator) into neurons, mediating its internalization in uninfected cells. Mutations in LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia.You may also be interested in the following LDLR products: Gene Expression Assays