|CD8α, Rat IgG2b monoclonal|
|Pacific Green™ dye|
Ex/Em and Laser Configuration:
|411/500 nm, violet laser|
Leukocyte Workshop Status:
Specificity for CD8α
The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/β heterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells. Mouse CD8α can also form a CD8α/β chain homodimer on subsets of CD8 positive T cells. For this reason, antibodies specific for CD8α rather than CD8β are recommended for a rigorous delineation of CD8 positive T cells. The 5H10 mAb has been used in other labs for flow cytometry and inhibition of T cell responses to IL-21.
Use the primary antibody selector tool to see the full list of CD8α antibodies.
You can get additional information about violet laser reagents in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.