|CD45R (B220), Rat IgG2a monoclonal|
|Pacific Green™ dye|
Ex/Em and Laser Configuration:
|411/500 nm, violet laser|
Leukocyte Workshop Status:
Specificity for CD45R
The RA3-6B2 monoclonal antibody (mAb) reacts with an exon A-specific epitope on CD45 that is expressed on essentially all B cells and is maintained throughout B cell development. The RA3-6B2 epitope is expressed in a restricted manner by B cells; however, it is also present on lymphokine activated killer (LAK) cells. Although B220 has been widely used as a pan B cell marker, CD19 may be a more appropriate pan B cell marker, as expression of CD19 appears to be more closely restricted to B cells. The RA3-6B2 mAb has been shown to potentiate isotype switching in B cells and to inhibit proliferative responses of B cell mitogens on B cells. Applications of RA3-6B2 include immunostaining for flow cytometry and immunoprecipitation.
Use the primary antibody selector tool to see the full list of CD45R antibodies.
You can get additional information about violet laser reagents in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.