Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer.
Bolt® Transfer Buffer can also be used with Bolt® Antioxidant
to enhance transfer of reduced proteins to membranes.About the Bolt® Mini Blot Module
The Bolt® Mini Blot Module
is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.About Bolt® Bis-Tris Plus Gels
Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Bolt® Mini Gel Tank
is required for use of all Bolt® gels.