This is a purified rabbit anti-human Insulin Receptor Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Insulin Receptor Polyclonal Antibody was produced in rabbits immunized with purified, recombinant human Insulin Receptor. Insulin Receptor specific IgG was purified by human Insulin Receptor affinity chromatograpy.
Antibody characteristics include:
Applications: This Insulin Receptor Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immunohistochemistry
Host species and isotype: This is a rabbit Polyclonal IgG against human Insulin Receptor
Immunogen: Recombinant human Insulin Receptor protein
Product size: 100 µg
INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).