|CD8α/Lyt-2, rat IgG2aκ monoclonal|
Ex/Em and laser configuration:
|488/767 nm, blue laser|
Leukocyte Workshop Status:
Specificity for CD8α/Lyt-2
This antibody is specific for mouse α chain of the murine CD8 heterodimer, Mr 32-34 kDa. In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an α chain (CD8α/Lyt-2) and a β chain (CD8β/Lyt-3); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
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