Proteinase K, FS
from the fungus Engyodontium album
is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K, FS is supplied specifically for use in food and environmental testing workflows. It is typically used in conjunction with Lysis Buffer, FS
(available separately) to lyse microbial organisms in order to prepare the microbial DNA for downstream analysis by real-time PCR.
, FS can be used as a part of both the Pathatrix® Auto Instrument and RapidFinder™ Direct Lysis workflows.
When used as a part of the Pathatrix® Auto Instrument workflow, one bottle of Proteinase K, FS
is sufficient for 1250 reactions.
When used as a part of the RapidFinder™ Direct Lysis workflow, one bottle of Proteinase K, FS
is sufficient for 100 reactions.
, FS is supplied in 50% glycerol at a concentration of 20 mg/ml.
One unit liberates 1 µmol of Folin-positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using denatured bovine hemoglobin as the substrate.