Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is:
- Gentle on cells
- Room-temperature stable
- Easy to use
- Animal origin-free (AOF)
This TrypLE™ Express is modified as follows:
|• Phenol Red|
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle On Cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.
TrypLE™ Express is room-temperature stable and ready to use. TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to Use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin-Free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.
cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® TrypLE™ Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.