Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
|Concentration||10 U/ µL|
|Contents||Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL) |
|Reaction Mixture ||Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL |
|Heat inactivation||Enzyme inactivated by heating at 65°C for 20 minutes.|
|Star Activity||Unrelated site may be cut in the presence of high concentration of glycerol.|
|Effect of DNA Methylation||Enzyme is affected by CG methylase.|
|Unit Definition||One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 30°C.|
|10X Buffer T|| 330 mM Tris-AC, pH 7.9 100 mM Mg-Ac 5 mM Dithiothreitol 660 mM K-Ac |
|Source||Serratia marcescens Sb|
Relative Activity in Universal Reaction Buffers
|Relative Activity (%)||<20||<20||<20||<20||100|
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.