Key product features:
- Achieve a standard measure for data comparison across gene expression experiments
- Measure sensitivity (lower limit of detection) and dynamic range of an experiment
- Quantitate differential gene expression
RNA analysis, including gene expression profiling and whole transcriptome surveying, can lead to better understanding of expression patterns in disease states and provides greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression. Traditional methods of RNA analysis, such as qRT-PCR and microarrays, are well established but are being replaced by next-generation sequencing, a high-throughput digital alternative. Because each method carries multiple platforms, and with the need to compare various samples across platforms throughout the world, a standard measure for data comparison is necessary. As the capabilities of RNA analysis expand, the necessity to create a standardized view of data will become even more important.
Achieve and Compare Results with Confirmed Accuracy
Ambion® ERCC RNA Spike-In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms. With two spike-in mix formulations (Figure 1), various measurements, such as sensitivity or dynamic range, can be examined to assess different parameters in an experiment or across experiments (Figure 2). Furthermore, expression fold-change ratios between two samples can be calculated with a high degree of confidence using the highly concordant relationship between ExFold RNA Spike-In 1 and ExFold RNA Spike-In 2 (Figure 3). Measurements are determined via known molar concentrations for each transcript within a spike-in mix and through association of the two mixes using a combination of ratios across 4 different subgroups of the 92 transcripts. The controls are ideal for next generation sequencing experiments, such as on SOLiD™ System, and supported microarray platforms such as the Illumina® Sentrix® BeadChip.
Choose Among Flexible Options for ERCC Kit Configurations
Whether measuring dynamic range or gene expression fold-change, Ambion® ERCC Spike-In Control Mixes are available in two kit configurations to meet your experimental needs. Use the ERCC Spike-In Mix to determine the dynamic range and lower limit of detection on your platform, and use the ERCC ExFold Spike-In Mixes to assess the accuracy of differential gene expression measurements.
|ERCC RNA Spike-In Mix 1*||ExFold Spike-In Mix 1*||ExFold Spike-In Mix 2*||Nuclease-free Water|
|ERCC RNA Spike-In Mix (Cat. No. 4456740)||10 µL||—||—||1.75 mL|
|ERCC ExFold RNA Spike-In Mixes (Cat. No. 4456739)||—||10 µL||10 µL||1.75 mL|
* Although ERCC RNA Spike-In Mix 1 and ExFold Spike-In Mix 1 contain the same formulation of ERCC transcripts, do not substitute ERCC RNA Spike-In Mix 1 for ExFold Spike-In Mix 1 for fold-change assessment. Use only ExFold Spike-In Mix 1 and Mix 2 with the same manufacturing lot number.
For Research Use Only. Not for use in diagnostics procedures.