This DMEM is modified as follows:
|• Low Glucose||• L-glutamine|
|• Sodium Pyruvate||• Phenol Red|
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (11880-028). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.