The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer I. It is also available with GeneAmp® 10X PCR Buffer II and MgCl2 Solution.
Also available is AmpliTaq® 360 DNA Polymerase for superior PCR results with the broadest range of templates. Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to help work through challenging GC-rich templates.