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Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands.
Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS.
Recommended Buffers: By choosing the appropriate Novex® pre-mixed buffer, you can create either native, denaturing or reducing running conditions with any Novex® Tris-Glycine Gel.
Wine Robert N; Dial John M; Tomer Kenneth B; Borchers Christoph H;
Anal Chem (2002) 74:1939-1945
Crosslinked and un-crosslinked samples were diluted 1:1 in Invitrogen non-reducing sample buffer, heated for 5 min at 56 C and then incubated at 22 C for 30 minutes before running on a gel. The gels used were 1 mm 10% Tris-glycine Invitrogen gels.
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