pVAX1 is specifically designed for use in the development of DNA vaccines. pVAX1 has the following features:
- Eukaryotic DNA sequences limited to those required for expression in order to minimize the possibility of chromosomal integration
- Kanamycin resistance gene for selection in E. coli because ampicillin has been reported to cause an allergic response in some individuals
- Expression levels of recombinant proteins from pVAX1 comparable to those achieved with its parent vector, pcDNA™3.1
The small size of pVAX1 and the variety of unique cloning sites simplify subcloning of even very large DNA fragments.