|• L-glutamine||• HEPES|
|• Phenol Red|
The complete formulation is available.
Using Ham's F-12K
Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Dual-site cGMP manufacturing
For supply chain continuity, Life Technologies manufactures Ham's F-12K (Kaighn's) Medium at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.