The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Size: 48 reactions
S100 Calcium Binding Protein A9 (S100A9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications This recombinant protein was expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114). Activity: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells.The ED 50 for this effect is typically 3-15 µg/ml. Formulation: Lyophilized in 140 mM NaC...
Size: 5 x 5 µg
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Size: 1 g
Myelin Oligodendrocyte Glycoprotein (MOG), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse MOG (Q61885) extracellular domain (Gly 29-Thr 156) fused to a C-terminal polyhistidine tag. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in PBS, pH5.48, 5% Mannitol, 5% Trehalose, 0.02% T...
Size: 5 µg
These individual EcoRI & MseI Adaptors can be used in the Restriction Ligation reaction. For Research Use Only. Not for use in diagnostics procedures.
Size: 100 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Size: 10 µg
The CaptureSelect™ IgG-CH1 affinity matrix recognizes the CH1 domain of human IgG antibodies, which enables purification of all subclasses (1, 2, 3 and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material. Due to its unique selectivity for the CH1 domain, no co-purification of free light-chain contaminants will occur. Since no cross-binding is observed for bovine IgG, the product can be used for the purification of IgGs and antibody fragments in the presence of fetal calf serum.
Size: 1 x 5 mL
TAOK3 (JIK) is a serine⁄threonine kinase that is a member of the GCK-like subfamily of the STE20 kinase family. JIK is ubiquitously expressed in human tissues, inhibits JNK and has been implicated in cancer.
Size: 1 mg
The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Size: 1 each
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Size: 5 nmol
CD45R (B220), clone RA3-6B2, Qdot® 800 nanocrystal conjugate is a rat monoclonal antibody (mAb) that recognizes the expressed product of the PTPRC gene. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD45R (B220), Rat IgG2a monoclonal Label: Qdot® 800 nanocrystal Validated Ap...
Size: 25 µL
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Opti-MEM® I modifications for a range of cell culture applications. This Opti-ME...
Size: 500 mL
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Size: 1 g
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Size: 1 kit
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Size: 1 kit
The LIVE/DEAD® Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer. • Stable—dyes are freeze dried in separate vials to maintain stability • Robust—staining pattern is the same before and after fixation • Bright signal—allows for easy distinction between live/dead cells in single channel Stable Unlike products that are so...
Size: 1 kit
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Size: 2,000 reactions
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Size: 500 g
The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate with Barcode is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR systems and thermal cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help ensure well-to-well temperature uniformity • E...
Size: 500 plates
The QuantStudio™ 3D Digital PCR UV Sealing Kit with Pen provides the materials needed to ensure an occlusive seal on the QuantStudio™ 3D Digital PCR Chip following reagent addition. This is accomplished through the application of sealant and the use of UV light to cure the sealant. This kit includes a syringe of sealant and a sealant tip, as well as a UV pen (batteries included) and pen holder. After loading sample onto the chip, apply the chip lid, add immersion fluid, and seal the fill-hole with the UV sealant and cure the sealant with the UV light pen. If you already have a UV pen and would...
Size: 1 kit
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Size: 1 x 96-well plate
The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product th...
Size: 50 reactions
The epidermal growth factor receptor is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics.
Size: 100 µg
These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market ( Features of TaqMan® assay beads: • Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required • Consistency—fewer operator steps mea...
Size: 96 reactions
The CellROX® Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Deep Red Reagent, as well as SYTOX® Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Deep Red Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines,...
Size: 100 assays
Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Size: 300 units
Optical adhesive film reduces the chance of well-to-well contamination and sample evaporation when applied to a microplate. • Pressure-sensitive adhesive provides a tight seal over every well • Will not interfere with sample reading • Press-n-seal: align properly to the plate, then press to seal • Easy to apply--will not stick to your glove Prevent Loss of Precious Samples Use this optically-clear adhesive film to seal the samples into the wells of a microplate. This will reduce the possibility of cross-contamination between sample wells and help ensure consistent real-time PCR data. Easy to U...
Size: 50 covers
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Size: 4 cards
The ProFlex™ 2x96-Well PCR System is the latest in our line of Applied Biosystems® thermal cyclers. The ProFlex™ system combines the reliability and performance you’ve come to expect from Applied Biosystems® instruments with the flexible configuration and control features that adapt to your research needs. Request a demo . The ProFlex™ 2x96-Well PCR System allows you to: • Interchange the 2x96-well, 0.2 mL block with another block type if your throughput needs change • Access the system remotely (and conveniently) through a mobile app • Program the instrument in seconds with a simple-to-use to...
Size: 1 instrument