Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Size: 1 x 5 ml (200 reactions)
Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes. • Processed faster than ultrafiltration columns • Rapid and efficient separations • Buffer is not preselected • Convenient 20-100 µL sample size • Dry gel format • Room Temperature Storage • Microcentrifuge format Designed for Per...
Size: 50 plates
Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Size: 10 x 5 ml, 2,000 reactions
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Size: 4 cards
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is i...
Size: 1,000 reactions
The DS-33 GeneScan™ Installation Standard consists of pooled PCR products labeled with 6-FAM™, VIC®, NED™ and PET® dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA has been amplified with 8 fluorescent PCR primer pairs which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan™ 600 LIZ™ Size Standard 2.0. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Size: 1 kit
The 21 CFR Part 11 Software Module for the 7500 Systems offers the flexibility of user-customizable security configuration settings to assist with 21 CFRp11 compliance. For Research Use Only. Not for use in diagnostics procedures.
Size: 1 kit
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
The Applied Biosystems® TaqMan® Mouse GPCR Array is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of mouse GPCR genes important in drug discovery. This product is designed with 380 TaqMan® Gene Expression Assays arrayed in singletons in 384 wells (plus 1 control arrayed in quadruplicate) and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include retinal receptors, small molecule receptors, chemokine receptors and others including classic endogenous control targets. This product is packaged in 1 box contai...
Size: 4 cards
The Applied Biosystems® MicroAmp® 96-Well Tray for VeriFlex™ Blocks is specifically designed to snugly hold MicroAmp® Fast Reaction Tubes with Caps and MicroAmp® Fast 8-Tube Strips (0.1 mL) in a 96-well format. They are also designed to snugly hold MicroAmp® Reaction Tubes with Caps (0.2 mL). Engineered for efficient sample processing Designed specifically for MicroAmp® Fast Reaction Tubes with Caps (0.1 mL) and MicroAmp® Fast 8-Tube Strips (0.1 mL) Designed specifically for MicroAmp® Reaction Tubes with Caps (0.2 mL) Manufactured to high specification, ensuring a quality product Convenience a...
Size: 10 trays
Ambion® Carrier RNA (1 mg⁄mL) is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one tube of 550 μL.
Size: 500 µl
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of this kit: • Fast —short reaction time (typically 30–60 min) • Convenient —easy workflow with few pipetting steps (2 tubes) • Reliable —reverse transcription of both abundant and limited targets • Flexible —optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction Streamline your cDNA synthesis while maintaining detection sensitivit...
Size: 50 reactions
The Silencer ® Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications for enhanced efficacy as in other Silencer ® Select siRNAs. Properties of Silencer ® Select control siRNAs: • Validated siRNA controls for optimizing siRNA experiments • GAPDH Positive Control functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® ...
Size: 5 nmol
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Size: 40 reactions
This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Size: 1 manual
The TotalPrep™-96 RNA Amplification Kit is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. Features of the TotalPrep™-96 RNA Amplification Kit: • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing set-up time and maximizing labeling Using the TotalPrep™-96 RNA Amplification Kit The TotalPrep™-96 RNA Amplification Kit incorporates extensive improvements to th...
Size: 96 reactions
For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Size: 1 set
The VLA TaqMan® Influenza A Detection Kit consists of a set of optimized reagents, enzymes, target-specific primers, and probes in a lyophilized reagent bead format. The lyophilized format provides a consistent and easy-to-use standard procedure for the rapid detection of viral nucleic acid derived from a variety of sample types. This kit, with the Influenza H7 and H5 Subtyping Kits, provides a consistent, rapid, and easy-to-use standardized procedure for detecting the presence of viral nucleic acid targets derived from a variety of samples. With the VLA TaqMan® Influenza A⁄H5⁄H7 Detection Kit...
Size: 1 kit
Borosilicate round bottom tubes (13 mm x 65 mm) and caps for use with the Covaris™ S2 System. For Research Use Only. Not for use in diagnostics procedures.
Size: 125 tube
PDGFRALPHA [pY742] PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PDGFRα [pY742]. This antibody is validated for use in Western Blot. Anti-PDGFRα [pY742] recognizes the expressed product of the Pdgfra gene.
Size: 10 blots
CK2BETA [pS209] PAb; Unconjugated Polyclonal antibody specific to Human, (Mouse, Rat, Chicken) CK2β [pS209]. This antibody is validated for use in Western Blot. Anti-CK2β [pS209] recognizes the expressed product of the CSNK2B gene. Catalog number 441090G is same antibody as former catalog number 441090. 441090G contains 50% glycerol, which improves the stability and longevity of this antibody.
Size: 10 blots
This rabbit anti-IR [pY972] polyclonal antibody is specific to human, mouse (rat) IR/IGFR [pY972]. Anti-IR/IGFR [pY972] polyclonal antibody recognizes the expressed product of the INSR gene. Biological actions of insulin are mediated by the insulin receptor (IR, ~95 kDa), a receptor tyrosine kinase that regulates multiple signaling pathways through activation of a series of phosphorylation cascades. The validated application for rabbit anti-IR [pY972] polyclonal antibody is Western blotting. Antibody Attributes: • Applications: The validated application for IR [pY972] polyclonal antibody is We...
Size: 10 blots
PKA REG 11BETA [pS114) PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PKA IIβ [pS114] (regulatory domain). This antibody is validated for use in Western Blot. Anti-PKA IIβ [pS114] (regulatory domain) recognizes the expressed product of the PRKAR2B gene. Catalog number 44998G is same antibody as former catalog number 44998. 44998G contains 50% glycerol, which improves the stability and longevity of this antibody.
Size: 10 blots
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Size: 1 ml, 400 reactions
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Size: 750 reactions
Ambion® Silencer ® SelectGAPDH Positive Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algo...
Size: 250 nmol
Hi-Di™ Formamide is a highly de-ionized formamide, formulated with a stabilizer, for use as an injection solvent on Applied Biosystems® 3500 Dx Series genetic analyzers. This highly deionized formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems and is appropriate for all applications. It is provided in a 5 mL bottle format to minimize freeze-thaw cycles.
Size: 5ml bottle
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Size: 4 tubes
Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications. • Low background fluorescence • High brightness in the presence of double-stranded DNA • Minimal temperature shif...
Size: 50 ml
This is a 96-well retainer & base set (standard) designed for use with the 3500 Dx⁄3500xL Dx Genetic Analyzer. Septa and plate base⁄retainer kits for 8 tube strip, 96 and 384-well plates used with the 3500 Dx Series Genetic Analyzers. Note: The 3500 Dx Series Systems and consumables are for distribution and use in select countries only.
Size: 4 sets