YES1 is a recombinant human protein kinase that is a member of the SRC family of non-receptor tyrosine kinases and is the cellular homolog of the Yamaguchi sarcoma virus oncogene. It is involved in the regulation of cell growth and survival, including roles in apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. • Active —this protein can be used in kinase activity assays, such as Z’-LYTE® assays, or in a binding assay platform like the LanthaScreen® Eu Kinase Binding Assay (KBA). • Purified —purity is determined by gel electrophoresis and provided in lot-specific certi...
Size: 1 mg
The CaptureSelect™ IgG-CH1 affinity matrix recognizes the CH1 domain of human IgG antibodies, which enables purification of all subclasses (1, 2, 3 and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material. Due to its unique selectivity for the CH1 domain, no co-purification of free light-chain contaminants will occur. Since no cross-binding is observed for bovine IgG, the product can be used for the purification of IgGs and antibody fragments in the presence of fetal calf serum.
Size: 1 x 5 mL
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 x 50 µL
The CaptureSelect™ C-tag Affinity Matrix combines unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. The CaptureSelect™ C-tag Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cytoplasm or periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity ...
Size: 5 mL
Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody is labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize cross-reactivity, it has been adsorbed against mouse IgG, mouse serum, and human serum prior to conjugation.
Size: 0.5 mL
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Size: 1 x 10 L
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Size: 300 units
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
This software module expands the functionality of the QuantStudio™ 12K Flex software, allowing high resolution melting (HRM) analysis to be performed on PCR amplicons. High Resolution Melt (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. Simple and fast, this method is based on PCR melt (dissociation) curve techniques and is enabled by the recent availability of improved double-stranded DNA (dsDNA)-binding dyes along with next-generation real-time PCR instrumentation and analysis software. HRM analysis can discriminate DNA sequ...
Size: 1 kit
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Size: 500 g
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Size: 1 L
Cystatin SA (CST2), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human CST2 (NP_001313.1) (Met 1-Ala 141) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in sterile double dis...
Size: 5 x 50 µg
Peptidyl Arginine Deiminase, Type Iv (PADI4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human PADI4 (NP_036519.2) (Met 1-Pro 663) fused to the N-terminal polyhistidine-tagged GST tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophilized in 20 mM Tris, 500 mM NaCl, 0.5...
Size: 5 x 50 µg
Membrane Metallo-Endopeptidase (MME) (CD10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of human CD10 (NP_000893.2) fused to the Fc region of human IgG1 at the N-terminus. Activity: Measured by its ability to cleave the fluorogenic pep...
Size: 5 x 50 µg
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
The epidermal growth factor receptor is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics.
Size: 100 µg
The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Size: 48 reactions
Meprin 1 alpha (MEP1A), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse MEP1A (NP_032611.2) (Met 1-Arg 615) fused with a polyhistidine tag at the C-terminus. Activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >400 pmoles/min/µg . Formulation: Lyo...
Size: 5 x 50 µg
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg
STK25 (YSK1) is a serine⁄threonine kinase that belongs to the GCK family of kinases. It has been implicated as an upstream regulator of MAP kinase signaling pathways.
Size: 100 µg
NEK4 is a serine⁄threonine kinase involved in signal transduction and cellular communication. It is proposed to act exclusively upon threonine residues and is present in most primary carcinomas.
Size: 100 µg
FGFR3 is a member of the FGFR family of receptor tyrosine kinases. This family is known to regulate a host of cellular functions including angiogenesis, mitogenesis, osteogenesis, myogenesis, carcinogenesis, cellular differentiation, and tissue repair after injury. The FGFR family has also been implicated in a number of diseases including cancer, rheumatoid arthritis, and diabetic retinopathy. The K650E mutation leads to constitutive activation and the neonatal lethal disorder TDII.
Size: 100 µg
Eukaryotic Translation Initiation Factor 2C 3 (EIF2C3), recombinant human protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human EIF2C3 (Q9H9G7-1) (Met 1-Ala 860) fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No rec...
Size: 5 x 50 µg
The Applied Biosystems® MicroAmp® Fast Optical 96-Well Reaction Plate is an integral component of the Applied Biosystems® Fast PCR Systems, which reduces PCR reaction time from 2 hours to as little as 25 minutes. • Maximum thermal conductivity for precise thermal cycling • Reproducible, specific, and sensitive PCR results in just 25 minutes • Validated with other system components for consistent results Increased Thermal Contact for Faster, More Uniform Heating The advanced design of the MicroAmp™ Fast 96-Well Reaction Plate dramatically increases its thermal contact with the Applied Biosystem...
Size: 10 plates
The highly deionized (Hi-Di) Formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems. The smaller configuration (5 ml) minimizes the number of freeze⁄thaw cycles compared to the larger 25ml bottle and may be a better fit when running a lower number of samples. For Research Use Only. Not for use in diagnostics procedures.
Size: 4 x 5 mL
Interleukin 1 Beta (IL1B), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of Cynomolgus (Macaca fascicularis) IL1B (Ala 117-Ser 269) fused to an initial Met. Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is typically 5-40 pg/ml. Formulation: ...
Size: 5 x 50 µg
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Size: 1 x 96-well plate
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Size: 1 g
Sulfotransferase family cytosolic 2B member 1 (SULT1B1), recombinant human protein is supplied in frozen format. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human SULT1B1 (NP_055280.2) (Leu 2-Ile 296) fused to a polyhistidine tag at the N-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 0.1 M NaCl, 10% glycerol, 1 mM DTT, pH 8.0. Reconstit...
Size: 5 x 50 µg
The Applied Biosystems® QuantStudio™ 3D Digital PCR Tilt Base for GeneAmp® PCR System 9700 sits beneath the GeneAmp® 9700 thermal cycler and ensures consistent performance during the PCR amplification of the QuantStudio™ 3D Digital PCR Chips. Simply place the Tilt Base beneath the feet of the thermal cycler, load your prepared 3D digital PCR chips, and begin PCR amplification.
Size: 1 base