The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Size: 40 reactions
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Size: 2 × 1 ml
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Size: 1 fast plate
The TaqMan® Array Human MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situatio...
Size: 4 pack
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Size: 1 standard plate
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Endogenous Control 96-well Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. The plate contains 16 commonly used housekeeping genes. The panel also contains 16 mouse orthologs of hum...
Size: 1 fast plate
The Applied Biosystems® TaqMan® Mouse GPCR Array is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of mouse GPCR genes important in drug discovery. This product is designed with 380 TaqMan® Gene Expression Assays arrayed in singletons in 384 wells (plus 1 control arrayed in quadruplicate) and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include retinal receptors, small molecule receptors, chemokine receptors and others including classic endogenous control targets. This product is packaged in 1 box contai...
Size: 4 cards
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Size: 750 reactions
The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR). • Pre-designed primers and TaqMan® probe eliminate assay design. • Rapid assay development guidelines can minimize optimization time. • Separate components provide flexibility in assay set-up. Comprehensive Guidelines Included Life Technologies® has developed a comprehensive set of ...
Size: 1 kit
The TaqMan® Fast Reagents Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5’ nuclease assay, in fast thermal cycling mode. The Eukaryotic 18S rRNA TaqMan® Assay consists of a 20X mix of two unlabeled PCR primers and a FAM™ dye labeled 18S TaqMan® MGB probe. Used With: 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module, 7900HT Fast Real-Time Service Upgrade, 7900HT System Fast 96-Well Block Upgrade Kit. For Research Use Only. Not for use in diagnostics procedures.
Size: 125 reactions
TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. The optimized formulation contains AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX™ dye serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on A...
Size: 2 packs, 500 reactions
The Applied Biosystems® TaqMan® GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your real-time PCR reactions. The kit includes human control RNA, GAPDH TaqMan® probe (JOE™ dye-labeled probe), and GAPDH primers. You can use either DNA or RNA as a target template. • Pre-designed primers and JOE™ dye-labeled TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehen...
Size: 100 reactions
This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Size: 1000 reactions
The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA. A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Size: 200 reactions
This kit contains the necessary accessories to process 10 TaqMan OpenArray® Real-Time PCR plates. TaqMan® OpenArray® Real-Time PCR Plates deliver world-class TaqMan® Assays in a more flexible, high-throughput format. Five customizable plate formats allow you to select from over 61,000 inventoried TaqMan® Gene Expression Assays, which are pre-loaded onto the OpenArray® Plates. View the available formats (PDF). The OpenArray® Real-Time PCR System enables over 8,000 real-time PCR assays to be run simultaneously to generate 32,000+ data points per day. As part of our greener product alternatives w...
Size: 1 kit
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Size: 10 packs, 2,000 reactions
TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT) 16 , and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents: • Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT) • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Using TaqMan® Reverse Transcription Rea...
Size: 1 pack, 200 reactions
The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Size: 1000 reactions
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Size: 1000 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instru...
Size: 1 x 5 ml, 200 reactions
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Size: 2 x 5 ml, 400 reactions
This mouse anti-Tau [pT231] monoclonal antibody is specific to human Tau [pT231]. Mouse anti-Tau [pT231] monoclonal antibody recognizes the expressed product of the MAPT gene. Clone PHF-6 recognizes phosphorylation dependent epitopes of Thr-231 on PHF-τ as well as fetal tau, but does not recognize autopsy-derived normal adult tau. Validated applications for mouse anti-Tau [pT231] monoclonal antibody are Western blot and ELISA. • Applications: Validated applications for mouse anti-Tau [pT231] monoclonal antibody are Western blot and ELISA. • Host species and isotype: The host species and isotyp...
Size: 50 µg
Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Size: 4 x 1 L
The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the ...
Size: 0.5 mg
Contains 50 mg TET™ Dye Phosphoramidite. TET™ Dye Phosphoramidite is used for the 5’ attachment of TET™ dye during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesis • Life Technologies offers a range of specialty phosphoramites and linkers...
Size: 50 mg
The Texas Red-X goat anti—rabbit IgG is labeled with our bright, photostable, red-fluorescent dye (which contains an additional seven-atom aminohexanoyl spacer ("X") between the fluorophore and the antibody). The antibody is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Size: 0.5 mL
Tfi DNA Polymerase is a new non- Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq , Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a co...
Size: 5,000 reactions
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Size: 50 ml
TIMP metallopeptidase inhibitor 1 (TIMP1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of human TIMP1 (NP_003245.1) (Cys 24-Ala 207). Activity: Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH 2 . The IC 50 value is approximately 6 nM. Formulation:...
Size: 5 µg
TMB (3,3’, 5,5;-tetramethylbenzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs. Using TMB Use this reagent as supplied, no dilution or further preparation is required. In the presence of peroxidase (HRP, POD), TMB will be converted to a blue color that can be read at OD 650 nm.
Size: 500 ml
Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 49) of mouse BCMA (NP_035738.1) precursor fused to the Fc region of human IgG1 at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophil...
Size: 5 x 5 µg
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Size: 20 reactions
The Total Exosome Isolation Kit (from plasma) enables fast and efficient enrichment of intact exosomes from plasma samples. The kit consists of two parts: Total Exosome Isolation Reagent (from plasma) and Proteinase K (20 mg/mL). The Total Exosome Isolation Reagent enables simple and reliable concentration of intact exosomes from plasma samples with a protocol that is scalable depending on your sample size. Proteinase K is provided separately for optional upfront plasma treatment and is used to eliminate all plasma protein and thus maximize the purity of the exosome preparation. • Maximize rec...
Size: 6 ml
Total Exosome Isolation Reagent (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation Reagent (from cell culture media) enables simple and reliable concentration of intact e...
Size: 50 ml
The TrackIt™ Loading Buffers are a 6X solution used for easy loading and tracking of DNA samples in agarose gels. These loading buffers contain two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples. TrackIt™ Cyan/Orange Loading Buffer is recommended for DNA f...
Size: 3 × 0.5 ml
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRIzol® LS Reagent: • Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein...
Size: 200 ml
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient than wild...
Size: 50 reactions
Ubiquitin-Conjugating Enzyme E2W (Putative) (UBE2W), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2W (Q96B02-12) (Met 1-Cys 151) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water to a...
Size: 50 µg
UltraPure™ 1 M Tris-HCl Buffer, pH 7.5, is a premixed, pH-adjusted, sterile-filtered solution. Prepared as a 1 M concentrate, this buffer can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and quality testing No DNase, RNase, or protease activity detected.
Size: 1 L
UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water. • Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels • Versatile: Detect DNA or RNA • Easy to Use: Can be used as-is or diluted to the desired concentration UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm ...
Size: 10 ml
UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Size: 1 L
UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to >20 kb. Features of UltraPure™ Agarose: • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved packaging The new environ...
Size: 100 g
Ambion® Ultrapure BSA is a "non-acetylated" BSA pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. In northern, Southern, and dot blot hybridizations, BSA is also used as a blocking agent. It is recommended in buffers for nick translation, polymerase reactions, and ligations. BSA is also a common additive for PCR amplifications, footprinting, and gel shift assays. In restriction digests, BSA has been shown...
Size: 1 x 50 mg
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Size: 1 L
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. Benefits of using UltraPure™ Low Melting Point Agarose: • Ideal for analysis and recovery of DNA and RNA for routine appli...
Size: 100 g
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. Using Phenol:Chloroform:Isoamyl Alcohol When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated a...
Size: 100 ml
This is a static, non-expiring license for Vector NTI® Express Designer that is intended for industrial users. For maximum benefit of this license, we recommend purchase of an Advanced Support Agreement (A15316). Vector NTI® Express Designer takes you beyond traditional sequence analysis and design and into the new frontier of rational bio-design. Vector NTI® Express Designer was designed for synthetic biology, metabolic engineering, genetic engineering, and molecular biology professionals who want to rationally design, characterize, assemble, re-factor, and reuse genetic "parts," "devices," a...
Size: 1 each
Versene is an EDTA solution for use as a gentle non-enzymatic cell dissociation reagent. Gibco® Versene Solution (0.48 mM) is formulated as 0.2 g EDTA(Na 4 ) per liter of Phosphate Buffered Saline (PBS). Product Use For Research Use Only. Not for use in diagnostic procedures. Dual-site cGMP Manufacturing and Quality System Gibco® Versene Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an...
Size: 100 mL
This kit allows the ViiA™ 7 Real-Time PCR System to operate using a Fast 96-well block. For Research Use Only. Not for use in diagnostics procedures.
Size: 1 upgrade
The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Size: 1 kit