StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Size: 500 ml
MitoTracker Green FM is green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. The dye will stain live cells but is not well-retained after aldehyde fixation.
Size: 20 × 50 µg
The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocel...
Size: 1 unit
This high-purity Ambion® Taq Polymerase is isolated from an E. coli strain that over-expresses this gene. Its heat stability allows DNA polymerization in the 5' to 3' direction during repeated heat denaturation and reannealing steps in the presence of dNTPs, a DNA template, and complementary primers. It is supplied in one tube containing 250 U at a concentration of 5 U/ µL. It can be used in PCR and RT-PCR reactions. Unit Definition: One unit incorporates 10 nmol of deoxynucleotides into acid insoluble material in 30 min at 74°C. SuperTaq ™ is a trademark of, and is manufactured by Enzyme Tech...
Size: 250 units
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Size: 1 g
EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one KOVA® Glasstic® Slide 10. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy. Note: an adapter is needed for use of this holder on the EVOS® FL Auto autom...
Size: 1 each
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of this kit: • Fast —short reaction time (typically 30–60 min) • Convenient —easy workflow with few pipetting steps (2 tubes) • Reliable —reverse transcription of both abundant and limited targets • Flexible —optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction Streamline your cDNA synthesis while maintaining detection sensitivit...
Size: 50 reactions
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (V-22885), as a paste (N-22880) or as a solid (D-282).
Size: 25 mg
This Donkey Anti-Sheep IgG (H+L) Antibody, Alkaline Phosphatase (AP) Conjugate is prepared from antibodies that have been adsorbed against human, mouse, and rabbit IgG to minimize cross reactivity. Donkey Anti-Sheep IgG (H+L) Antibody, Alkaline Phosphatase (AP) Conjugate should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF). Applications: Donkey Anti-Sheep IgG (H+L) Antibody, Alkaline Phospha...
Size: 1 mg
Streptavidin-FITC conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunomicroscopy and fluorescence-activated cell sorting and analysis. The amount of conjugate provided is sufficient for 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.
Size: 50 µL
Activin Receptor IIB (ACVR2B), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applicationsThis recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Thr 134) from the extracellular domain of human ACVR2B (NP_001097.2) was expressed with the fused human IgG1 Fc region at the C-terminus. Activity: 1. Mea...
Size: 50 µg
The BenchMark™ Pre-Stained Protein Ladder is a standard used to monitor electrophoresis or electrophoretic transfer from gel to membrane in Western blots. It consists of 10 proteins ranging in apparent molecular weight from 6 to 180 kDa. It is designed for use with Tris-Glycine gels. The BenchMark™ Pre-Stained Protein Ladder offers: Proteins covalently bound to a blue dye for easy visualization One protein band coupled to a pink dye for easy orientation and protein identification Affinity-purified proteins to provide sharp bands and clear background Easy-to-use format for direct application to...
Size: 2 × 250 µl
Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Size: 400 ml
The CD45RA, clone HI100, PerCP-Cy® 5.5 tandem conjugate binds to human CD45RA protein. PerCP-Cy® 5.5 tandem conjugate combines the peridinin-chlorophyll proteins (PerCP) complexes found in nature with the Cy® 5.5 cyanine dye, thus permitting simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser. Antibody Specifications Description: CD45RA, Mouse IgG2b kappa monoclonal Label: PerCP-Cy® 5.5 tandem Validated Application: Flow Cytometry Species Reactivity: Human Clone ID: HI100 Ex/Em and laser configu...
Size: 25 tests
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg
Exosome Spin Columns (MW 3000) enable fast removal of unincorporated dye from exosome labeling reactions. They also allow the user to perform buffer exchange on the exosomes or to remove any low molecular weight (MW ≤ 3000) admixtures from the exosome preparation such as salts, nucleotides, and short oligonucleotides. • Fast, efficient removal of unincorporated dye from the labeled exosomes • Buffer exchange, desalting, removal of low molecular weight contaminants • Replaces more time-consuming purification methods (such as ultra-centrifugation) To use, simply rehydrate the spin column, centri...
Size: 30 columns
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer. Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verif...
Size: 9 units
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock ® Mini-Cell. Formulation Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS. Recommended buffers For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Fic...
Size: 1 box
NuPAGE® Novex® 4-12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional Tris-Glycine gels, NuPAGE® Novex® Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE® Novex® Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE® gels for optimal results during day-to-day use. NuPAGE® Novex® Bis-Tris gels come in two sizes and multiple well formats. ...
Size: 10 gels
The CD27, clone O323, APC (allophycocyanin) conjugate binds to human CD27 protein. APC is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of APC in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: CD27, Mouse IgG1 kappa monoclonal Label: APC (allophycocyanin) Validated Application: Flow Cytometry Species Reactivity: Human Clone ID: O32...
Size: 25 tests
Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 49) of mouse BCMA (NP_035738.1) precursor fused to the Fc region of human IgG1 at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: Lyophil...
Size: 5 x 5 µg
Immunogen: Mouse IgG2a Specificity: Mouse IgG2a. Exhibits no reactivity with other Mouse IgG subclasses, IgA, IgM, or light chains. Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Western blotting
Size: 0.45 mg
The CD117, clone 104D2, fluorescein (FITC) conjugate is a mouse monoclonal antibody that detects human and bovine CD117. Commonly used, the fluorescein conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Antibody Specifications Description: CD117, mouse IgG1 Label: Fluorescein (FITC) Validated Application: Flow Cytometry Species Reactivity: Human, bovine Ex/Em and laser configuration: 488/519 nm, blue laser Clone ID: 104D2 Leukocyte Workshop Status: N/A Regulatory Status: RUO
Size: 100 tests
Immunogen: Mouse IgA Specificity: Mouse IgA Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), WB
Size: 1 ml
The Applied Biosystems® QuantStudio™ 3D Digital PCR Chip is at the heart of the QuantStudio™ 3D Digital PCR System. Manufactured from a silicon substrate and etched with 20,000 consistently-sized nanoscale reaction wells, the chip enables precise sample loading with an uncomplicated workflow. When combined with the QuantStudio™ 3D Digital PCR instrument, software, and associated reagents, the chip is designed to deliver accurate, sensitive, and precise digital PCR data. Bring digital PCR into your lab with this 12-pack of QuantStudio™ 3D Digital PCR Chips, each sufficient for a single sample.
Size: 12 chips
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes™ Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Live ReadyProbes™ Reagent: Ready-to-use liquid formulation of high-purity Hoechst 33342 No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or cell cult...
Size: 1 kit
RPCI-11 HS BAC CLONE
This Donkey Anti-Rabbit IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate is prepared from antibodies that have been affinity purified. Donkey Anti-Rabbit IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF). Applications: Donkey Anti-Rabbit IgG (H+L) Antibody, Horseradish Peroxidase (HRP) Conjugate should be suitable for we...
Size: 1 mg
The EVOS® Onstage Incubator Gas Line connects the Control Unit and the Stagetop Environmental Chamber units of the EVOS® Onstage Incubator and helps precisely control the CO 2 and O 2 levels. When performing live cell imaging studies, it is essential that the environmental conditions are precisely controlled. The EVOS® Onstage Incubator has three gas line ports, enabling studies of cells under normal, as well as hypoxic, conditions. Explore the entire EVOS® line of imaging systems and accessories .
Size: 1 each
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Size: 100 mL
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 100 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, an...
Size: 100 ml
Replacement Parts for Electrophoresis Apparatus
Size: 1 each
This Goat Anti-Mouse IgG (H+L) Antibody, Unconjugated is prepared from antibodies that have been affinity purified. Goat Anti-Mouse IgG (H+L) Antibody, Unconjugated should be suitable for all standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF). Applications: Goat Anti-Mouse IgG (H+L) Antibody, Unconjugated should be suitable for all standard immunoassay applications. Host species: The host species of theanti-mouse IgG (H+L), unconjugated is goat. Reactivity: Goat ...
Size: 2 mg
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Size: 1 kit
The Applied Biosystems® MicroAmp® 96-Well Tray for VeriFlex™ Blocks is specifically designed to snugly hold MicroAmp® Fast Reaction Tubes with Caps and MicroAmp® Fast 8-Tube Strips (0.1 mL) in a 96-well format. They are also designed to snugly hold MicroAmp® Reaction Tubes with Caps (0.2 mL). Engineered for efficient sample processing Designed specifically for MicroAmp® Fast Reaction Tubes with Caps (0.1 mL) and MicroAmp® Fast 8-Tube Strips (0.1 mL) Designed specifically for MicroAmp® Reaction Tubes with Caps (0.2 mL) Manufactured to high specification, ensuring a quality product Convenience a...
Size: 10 trays
Fibroblast growth factor 1 (FGF1/aFGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Phe 16-Asp 155) of mouse FGF1 (NP_034327.1). Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED 50 for this effect is typically 0.05-0.25 ng/mL in the presence of 10 µg/ml heparin. Formulation: Lyophilized in 20 mM MES buffer, pH 6.0, 200...
Size: 5 µg
UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water. • Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels • Versatile: Detect DNA or RNA • Easy to Use: Can be used as-is or diluted to the desired concentration UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm ...
Size: 10 ml
DTT is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's DTT is 1M in water. It is tested for background fluorescence, making it optimal for use in high-throughput screening applications.
Size: 1 ml
Apolipoprotein E (APOE), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human APOE (P02649) (Lys 19-His 317) fused to a Trx and a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Formulation: PBS, 40% Glycerol, 0.5 mM TCEP, pH 7.4. Reconstitution: No reconstitution needed. Store...
Size: 5 x 5 µg
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Size: 10 mg
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Size: 500 ml
Replacement Parts for Electrophoresis Apparatus
Size: 1 each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM/F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Size: 500 ml
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. This Advanced DMEM/F-12 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Size: 500 ml
Same as TaqVet Neospora caninum Real-Time PCR Kit TaqMan® probes - DNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits)...
Size: 50 tests
The EVOS® FL Auto Imaging System is a fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system with outstanding workflow efficiency. Designed to meet demanding requirements over a broad range of applications, the EVOS® FL Auto system supports high-resolution mosaic tiling, multi-position well scanning, cell counting with thresholding, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage and parfocal optical system enables you to produce publication quality images in seconds. The EVOS® FL Auto...
Size: 1 each
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Size: 10 × 500 ml
Same as TaqVet Triplex bRSV & PI3 Real-Time PCR Kit TaqMan® probes - RNA - triplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA ki...
Size: 50 tests
The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. >> Download a 90-day trial of the QuantStudio™ 7 software The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-w...
Size: 1 system
The EVOS® XL Core Imaging System is a digital, transmitted light, inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its color camera and high-quality optical system deliver high-definition images with exceptional ease. With a small footprint and an ergonomic design, the all-in-one EVOS® XL system is ideal for basic imaging needs and a perfect addition to any cell culture room or facility. The EVOS® XL Core Imaging System offers you these important advantages: • Easy installation; no maintenance, assembly, alignment, or calibration • Configured for f...
Size: 1 each