These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Size: 1 x 96-well plate
LysoSensor™ Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pK a of LysoSensor Yellow/Blue DND-160 is ~4.2.
Size: 20 × 50 µl
Mouse anti-Maltose Binding Protein; Unconjugated Monoclonal antibody specific to Multiple Maltose Binding Protein. This antibody is validated for use in Western Blot, Immunoassay (ELISA). Anti-Maltose Binding Protein recognizes the expressed product of the MBP gene.
Size: 100 µg
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Size: 20 each
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides: • Rapid, non-hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and convenient column purification The PureLink® RNA Mini Kit provides rapid purification of total RNA from a...
Size: 50 preps
POP-6™ is a denaturing polymer used on the 310 Genetic Analyzer. POP-6™ Polymer is used for sequencing applications. • Easy to Use: Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation. • Robust Formulation: Formulated for sequencing and fragment analysis applications. • Replenishable: Allow capillaries to be used multiple times. Reproducible Results POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP Po...
Size: 3 ml
The HiMark™ Unstained High Molecular Weight (HMW) Protein Standard is designed for analysis of high molecular weight proteins on Novex® NuPAGE® Tris-Acetate Gels . • Comprehensive – Contains markers with a wide range of high molecular weights, providing 9 protein bands in the range of 30-460 kDa • Convenient – A ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use • Compatible – Intended for use with NuPAGE® Novex 3-8% and 7% Tris-Acetate Gels under denaturing conditions* *Note: HiMark™ Unstained High Molecular Weight Protein Standard can also be used...
Size: 250 µl
The phycobiliprotein, allophycocyanin (APC) can be conjugated to proteins such as antibodies and other molecules to produce conjugates with excitation/emission maxima ~650/680 for use in flow cytometry applications. The Protein-Protein Crosslinking Kit (P6305) is optimized to crosslink APC to IgG antibodies.
Size: 0.5 mL
The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.
Size: 100 µl
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells: • Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal • Maintains plasmid DNA with minimum recombination events • Delivers high-yield plasmid preparations for downstream applications • M13mp cloning vectors may be propagat...
Size: 10 reactions
This Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) Antibody is highly cross-adsorbed and labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum.
Size: 0.5 mL
This Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) Antibody is highly cross-adsorbed and labeled with our superior red-fluorescent Alexa Fluor® 594 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG.
Size: 0.5 mL
Alexa Fluor® 488 Donkey Anti-Goat IgG (H+L) Antibody is labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from goat. To minimize cross-reactivity, it has been adsorbed against rabbit, rat, mouse, and human IgG.
Size: 0.5 mL
CellEvent® Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent® Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death. Like other ReadyProbes™ reagents, CellEvent™ Caspase-3/7 Green reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent provides...
Size: 1 kit
Image-iT® FX Signal Enhancer ReadyProbes™ Reagent dramatically reduces background fluorescence resulting from non-specific staining during secondary detection using conjugates of streptavidin or secondary antibodies. This reagent is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes. Features of Image-iT® FX Signal Enhancer ReadyProbes™ Reagent include: • Easy protocol—apply ready-made solution using a dropper bottle • Compatible with the other steps in your immunostaining protocol • Effective with most f...
Size: 1 kit
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextran Specifications : Label (Ex/Em): Rhodamine B (570/590) Size: 70,000 MW Charge: Zwitterionic Fixable: Nonfixable High Manufacturing Standards of Molecular Probes® Dextrans We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrop...
Size: 25 mg
NuPAGE® Novex® 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-...
Size: 1 box
Alexa Fluor 647 F(ab') 2 fragment of goat anti—rabbit IgG is labeled with our exceptionally bright, far-red—fluorescent Alexa Fluor 647 dye (which is spectrally similar to Cy5 dye) and is prepared from antibodies that have been adsorbed against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins to minimize crossreactivity. Conjugates of F(ab') 2 fragments are sometimes preferable to whole antibody conjugates for secondary detection, since the absence of the Fc region in F(ab') 2 fragments prevents interactions with Fc receptor-bearing membran...
Size: 250 µl
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Size: 1 mg
Immunogen: Human IgG Specificity: Human IgG (H+L) Reactivity: Human IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB ZyMAX™ antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX™ conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background....
Size: 1 ml
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Size: 10 g
Invitrogen offers a variety of tips for easy and accurate loading of samples. They are RNase/DNase-free and non-autoclavable.
The MHC Class I (H-2Kb), clone AF6-22.214.171.124, APC (allophycocyanin) conjugate binds to the mouse MHC Class I protein, H-2Kb. APC is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of APC in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: MHC Class I (H-2Kb), Mouse IgG2a kappa monoclonal Label: APC (allophycocyanin) Validated Applicatio...
Size: 25 µg
The Homogenizer is used with the Micro-to-Midi™ Total RNA Purification System to reduce the viscosity of difficult tissue samples between 5 and 30 mg in RNA isolation procedures.
Size: 1 each
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Size: 20 reactions
Reactivity: FITC-Avidin reacts with biotin.
Size: 5 mg
These combs are for use when pouring your own special gel types and are available in one thickness (1.0 mm) and two well formats (20 well and 26 well). They are compatible with our midi-size Empty Gel Cassettes (WC1012).
Size: 1 pack
Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Size: 100 ml
S100 Calcium Binding Protein A8 (S100A8), recombinant human protein is supplied in frozen format. It is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human S100A8 (NP_002955.2) (Leu 2-Glu 93) fused to a polyhistidine tag at the N-terminus. Activity: Measured by its ability to bind recombinant human S100...
Size: 5 µg
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Size: 10 ml
The Applied Biosystems® MicroAmp® Fast 96-Well Tray, specifically designed to snugly hold MicroAmp® Fast Reaction Tubes with Cap (0.1 mL) and MicroAmp® Fast 8-Tube Strips, is required for more efficient sample processing on the Applied Biosystems® 9800 Fast Thermal Cycler. • Organizes your Fast Reaction Tubes or Strips • Provides the thermal contact necessary for fast PCR • Manufactured to high specification, ensuring a quality product Convenience and Performance The MicroAmp® Fast 96-Well Tray allows you to organize your tubes while adding sample and mix. This handy tray reduces spillage and ...
Size: 10 trays
The iBind™ Solutions Kit is intended for use with the iBind™ Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind™ solutions act as the blocking, washing, and dilution solutions in the iBind™ immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind™ device wells to the iBind™ Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allo...
Size: 1 kit
Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES (Embryonic Stem Cells) and iPS (Induced Pluripotent Stem Cells) in culture. It directly replaces FBS in existing protocols. One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to ...
Size: 100 ml
This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avidin and Biotin solutions.
Size: 1 kit
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Size: 0.5 mL
Molecular biology grade, Ambion® 20X SSC solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating non...
Size: 500 ml
The GlobalFiler™ Express Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Express Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, p...
Size: 1,000 reactions
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Size: 100 mL
Same as TaqVet West Nile Virus - Dual IPC - Real-Time PCR Kit (WNPEXO/50) TaqMan® probes - RNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT en...
Size: 50 tests
Building upon the proven reliability and performance of the Applied Biosystems® 9600 and 9700 GeneAmp® Thermal Cycler instruments, the HID Veriti® Thermal Cycler features a 96-well (0.2 mL) format with six independent temperature zones to provide precise temperature control and rapid temperature changes for conducting polymerase chain reactions (PCR). Each HID Veriti® 96-Well Thermal Cycler features a powerful color touch screen and a USB port to simplify instrument setup and use. Proven Reliability Validated for use with four-dye, five-dye, and six-dye AmpFlSTR® PCR amplification kits, the HI...
Size: 1 instrument
The 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications. • Perform comparative sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection, and many other applications. • Eliminate gel preparation. • Set up runs in minutes. • Automate your workflow to minimize hands-on time and costs. • Detect five dyes simultaneously to increase throughput. Versatile, Affordable, Easy-to-Use, and Almost Hands-Free The 310 Genetic Analyzer – the ideal alternative to gel-base...
Size: 1 instrument
The 3500 Dx Genetic Analyzer CS2 is the latest addition to our family of Applied Biosystems® genetic analyzers based on capillary electrophoresis. The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled instrument, making it the first Sanger sequencing platform cleared for sequencing analysis of DNA. Confidence in Your Results • The 3500 Dx Genetic Analyzer CS2 is an IVD-labeled platform for Sequencing-Based Typing using SeCore® HLA typing kits. Sanger sequencing is known as the “gold-standard” for its accuracy, reliability, and ease of use. It is also used for confirmatory sequencing by clinical l...
Size: 1 system
Same as TaqVet BVDV Tissue Real-Time PCR Kit (BVD-TISSUE) TaqMan® probes - RNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for R...
Size: 100 tests