Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol).
Size: 25 mg
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Size: 750 reactions
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Size: 1 kit
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Size: 10 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Size: 100 mL
Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by dithiothreitol (DTT) .
Size: 1 g
Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Size: 1 ml
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Size: 2 × 1 ml
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Size: 100 mL
Novex® Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer applications using Tris-Glycine gels. Using premixed buffers Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.
Size: 500 ml
The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
Sodium pyruvate is commonly added to cell culture media as an energy source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, we recommend continuing use of the supplement as cell growth may lag without it. Gibco® Sodium Pyruvate (100 mM) is formulated using 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture media is 1 mM. Dual-site cGMP Manufacturi...
Size: 100 mL
This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Size: 233 ml
The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides: The araBAD promoter for tightly regulated expression Translation initiation signals optimized for E. coli expression N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody Enterokinase cleavage site for removing the N-terminal tag follo...
Size: 1 kit
The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Size: 1 kit
Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Size: 5 mg
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Size: 0.7 mg⁄ml
These products are advised for use with Dynal T4 Quant: Acridine Orange Staining Solution - for use with fluorescent microscopy. Sternheimer-Malbin Staining Solution - for use with light microscopy. Turck Staining Solution - for use with light microscopy. Washing/dilution Buffer - 20 X concentrated phosphate buffered saline (PBS) with 2% bovine serum albumin and 2% sodium azide.
Size: 1 unit
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
Size: 100 mg
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • L-glutamine • Sodium Pyruvate • Phenol Red • HEPES
Size: 1 L
BlueJuice™ Gel Loading Buffer (10X) is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels. Product use The recommended concentration of this buffer for use with all DNA samples run on agarose gels is 2X (one part buffer plus four parts sample). For acrylamide gels, the recommended concentration is 1X (one part buffer plus nine parts sample).
Size: 3 × 1 ml
This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Size: 1 manual
The Novex® Power Supply Adapters allow you to connect the covered power leads of electrophoresis units to certain power supplies (for example, some power supplies from Bio-Rad Laboratories, Thermo Fisher Scientific). The following electrophoresis units from Invitrogen have covered power leads to be in compliance with the latest IEC (International Electrotechnical Commission) and EN (European) standards, and reduce the risk of electrical shock due to exposure to bare metal. • ZOOM® IPGRunner™ Mini-Cell and ZOOM® IEF Fractionator • XCell4 SureLock™ Midi-Cell • XCell6™ MultiGel Unit • XCell SureL...
Size: 1 set
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Size: 5,000 reactions
• For use with SSP products or any application requiring agarose gel separation • Multiple formats available to meet various throughput needs • Includes replacement parts for each unit
Size: 1 each
The Primer Express® Software v3.0.1 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our StepOne®, StepOnePlus®, 7300, 7500, 7500 Fast, 7900HT, ViiA™ 7, and QuantStudio™ real-time PCR systems, Primer Express® Software provides customized application-specific documents for absolute⁄relative quantitation and allelic discrimination. • Enables individual oligonucleotide design for real-time PCR applications. • Supports assays based o...
Size: 1 license
The E-Gel® Safe Imager™ Real-Time Transilluminator allows you to watch migration of DNA in real-time in E-Gels containing SYBR® Safe. It offers: • Real-Time monitoring of DNA migration • Improved safety for the user • Enhanced cloning efficiency of retrieved DNA Real-Time Transilluminator Designed for Safety and Cloning The E-Gel® Safe Imager™ Real-Time Transilluminator is designed to fit perfectly beneath the E-Gel® iBase™ Power System and is intended for use with E-Gels containing SYBR® Safe. Blue light emitted by the instrument is ideal for viewing DNA that is stained with SYBR® Safe. Unlik...
Size: 1 each
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
The SuperScript® First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 µg of total RNA. After synthesis, the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations. In conjunction with PCR, the system can be used to detect the presence of rare messages, to quantitate the amount of specific mRNA from small numbers of cells, or to clone specific cDNAs without constructing an entire cDNA library. The syste...
Size: 50 reactions
ADP-ribosyltransferase 4 (ART4), recombinant mouse protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse ART4 (EDL10575.1) (Met1-Lys 263) fused to a C-terminal polyhistidine tag. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80. Reconstitution: Dissolve the protein in...
Size: 50 µg
The Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation. How it works The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate. Calcium phosphate facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis. The method was first developed by Graham and van der Ebb and was later modified by Wigler. The procedure is routinely used to transfect a wide variety of cell types f...
Size: 75 reactions
RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA; it is used for the isolation of RNA-free DNA. Concentration 20 mg RNase A/mL in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA. Specifications An essentially protease-free, freeze-dried powder with an activity of not less than 70 U/mg material.
Size: 25 ml
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Size: 1 mg
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µl
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Size: 1 kit
These individual EcoRI & MseI Adaptors can be used in the Restriction Ligation reaction. For Research Use Only. Not for use in diagnostics procedures.
Size: 100 reactions
Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Size: 500 g
Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.
Size: 500 ml
Hi-Di™ Formamide is a highly de-ionized formamide, formulated with a stabilizer, for use as an injection solvent on Applied Biosystems® 3500 Dx Series genetic analyzers. This highly deionized formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems and is appropriate for all applications. It is provided in a 5 mL bottle format to minimize freeze-thaw cycles.
Size: 5ml bottle
Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus . Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco® Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and is used for the prevention of bacterial contamination of cell cultures. This product is provided as a 10 mg/ml stock solution. The recommended working concentration for cell culture is 100 µg/ml. Life Technologies offers a variety of antibiotics an...
Size: 100 ml
The MicroAmp® Cap Installing Tool helps you install strips of MicroAmp® caps onto MicroAmp® reaction tubes in a tray⁄retainer set or 96-well reaction plate. • Available in handle style • Seal 8 or 12 cap strips • Recessed cup for pressing caps onto tubes • Convenient tool ensures proper sealing Press and Seal Caps The MicroAmp® Cap Installing Tool enables you to ergonomically seal MicroAmp® reaction tubes and plates. This tight seal will prevent well-to-well contamination and sample loss. Used With MicroAmp® Optical 8-Cap Strip, MicroAmp® Optical 8-Tube Strip, 0.2 ml, MicroAmp® Optical 96-Well...
Size: 1 tool
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family.
Size: 1 mg
The pcDNA™3.3-TOPO® TA Cloning® Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits: • TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-earl...
Size: 20 reactions
One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. One Shot® TOP10 cells: • Maximize cloning efficiency in a single-tube format • Provide enhanced genomic DNA cloning capabilities Easy-to-use One Shot® format The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in th...
Size: 40 reactions
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Size: 20 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Borosilicate round bottom tubes (13 mm x 65 mm) and caps for use with the Covaris™ S2 System. For Research Use Only. Not for use in diagnostics procedures.
Size: 125 tube
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Size: 2 x 2,500 units
One Shot® BL21(DE3) Chemically Competent E. coli are ideal for use with bacteriophage T7 promoter-based expression systems (e.g., pRSET, pCR®T7, and pET). BL21(DE3) cells carry the lambda DE3 lysogen. Recombinant proteins that are nontoxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE. About BL21 strains BL21 strains are descended from the E. coli B strain and have been specifically construct...
Size: 20 × 50 µl
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency® Stbl2™ Competent Cells offer: • >1 x 10 9 transformants/µg plasmid DNA for cloning rare sequences • Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A—allows cloning of methylated genomic sequences • A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV ...
Size: 10 reactions