Fungizone® Antimycotic is a formulation of amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® Antimycotic is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco® Fungizone® contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/mL. Life Technologies offers a wide range of antibiotics and ...
Size: 20 mL
Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Size: 25 mL
S100 calcium binding protein B (S100B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) fused to the Fc region of human IgG1 at the N-terminus. Activity: Measured in a functional ELISA: 1. Measured by its ability to bind mouse S100A1. 2. Measured by its ability to bind TP53. Formulatio...
Size: 5 µg
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Size: 80 µL
Replacement Parts for Electrophoresis Apparatus
Size: 1 each
Use Applied Biosystems® MicroAmp® Adhesive Film Applicator to form a tight seal between a microplate and an adhesive film. Form a Tight Seal The MicroAmp® Adhesive Film Applicator enables you to ergonomically push an adhesive film down upon a microplate to form a tight seal and minimize air bubbles. This tight seal will prevent well-to-well contamination and sample loss. Quick and Easy Use the MicroAmp® Adhesive Film Applicator to speed the process of sealing multiple microplates. This tool is designed according to the microplate dimensions so you can quickly seal a microplate. The rugged prop...
Size: 5 applicators
Borosilicate round bottom tubes (13 mm x 65 mm) and caps for use with the Covaris™ S2 System. For Research Use Only. Not for use in diagnostics procedures.
Size: 125 tube
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Size: 500 assays
Normal goat serum. Whole serum is obtained from non-immunized (preimmune) healthy goats. The serum is clarified through a 0.8 micron low protein binding filter.
Size: 5 mL
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisia e. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance.
Size: 20 µg
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Size: 5 bottle
The PureLink® Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ~30 minutes using PureLink® silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum. Advantages of using PureLink® Quick Gel Extraction Kit: • Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points • Complete the procedure in ~30 minutes ...
Size: 50 preps
Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Size: 3 mg
The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Size: 1 each
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero. This Advanced DMEM is manufactured as follows: With Without • High glucose • L-glutamine • Non Essential Amino Acids • Sodium pyruvate • Phenol Red
Size: 500 mL
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer. Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verif...
Size: 9 units
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Tri Reagent® solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. Key features: • Simple —a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive —obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile —works with many sample sources, including human, plant, yeast, bacterial and viral samples • Flexible —easily scalable depending on starting material and desired yield Using TRI Reagent® solution TRI R...
Size: 100 mL
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Size: 20 µg
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells: • Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal • Maintains plasmid DNA with minimum recombination events • Delivers high-yield plasmid preparations for downstream applications • M13mp cloning vectors may be propagat...
Size: 10 reactions
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Size: 1 kit
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Size: 1 kit
Immunogen: Mouse IgM Specificity: Mouse IgM Reactivity: Mouse Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Size: 1 mL
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Size: 40 reactions
In addition to data and software, Compendia Bioscience™ custom services are offered to support customers at various stages of drug discovery research. Onsite training is a service offering where a Compendia scientist visits a customer site for a comprehensive product overview, live demo, and one-to-one training/coaching sessions for prospective and current customers. Please contact us for more information.
Size: 1 each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM/F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Size: 500 mL
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Size: 50 mL
This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on an external publication. The assay has been optimized for use with the included Environmental Master Mix; a negative contro...
Size: 100 reactions
Cytochalasin D is a cell permeable fungal toxin that binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits. This compound causes the disruption of actin filaments and inhibition of actin polymerization. Format/Formulation: Lyophilized
Size: 1 mg
The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2). The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of ...
Size: 20 µg
Propidium iodide is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.
Size: 10 mL
Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. Dual-site cGMP Manufacturing and Quality System Gibco® HE...
Size: 20 x 100 mL
Fc gamma receptor II-b (FCGR2B/CD32b), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Ala 46-Pro 217) of mature human FCGR2B (NP_001002274.1) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag. Activity: Measured by its binding ability in a functional ...
Size: 5 x 5 µg
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µL
Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Size: 18 mL
This is an affinity-purified rat monoclonal antibody against mCherry Fluorescent Protein. The full-length protein was used as the immunogen and the resulting monoclonal IgG2a-isotype antibody detects denatured and native forms of mCherry. Recommended for use in: • Western analysis • Immunocytochemistry • Immunoprecipitation • Flow cytometry • Immunohistochemistry Because of its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes a...
Size: 100 µL
A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications. Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection • Specially optimized for use with live cell expe...
Size: 25 each
Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Size: 1 mg
Ambion® Silencer ® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using oth...
Size: 5 nmol
This kit allows the ViiA™ 7 Real-Time PCR System to operate using a TaqMan® Array Block. For Research Use Only. Not for use in diagnostics procedures.
Size: 1 upgrade
The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Size: 1 kit
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Size: 1 each
Xpress Mouse Monoclonal Antibody is designed to specifically interact with the Xpress™ leader peptide ( -Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys- ). Antibody specifications • Applications —western blotting, immunoprecipitation, immunofluorescence, and ELISA • Host species and isotype —mouse IgG1 • Product size —supplied in a 50 µL pack size
Size: 50 µL
The NativePAGE™ Novex® Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analysis of native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessment of purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow (1-3). With the NativePAGE™ Novex® Bis-Tris Gel System, youll: • Simplify resolution of large proteins (15-10,000 kDa) • Analyze membrane-protein complexes in their native conformations • ...
Size: 1 box
Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. • Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR • The optimized formulation contains highly purified AmpliTaq Gold® D...
Size: 1 x 5 mL (200 reactions)
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Size: 20 mL
Ambion® Ultrapure BSA is a "non-acetylated" BSA pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. In northern, Southern, and dot blot hybridizations, BSA is also used as a blocking agent. It is recommended in buffers for nick translation, polymerase reactions, and ligations. BSA is also a common additive for PCR amplifications, footprinting, and gel shift assays. In restriction digests, BSA has been shown...
Size: 1 x 50 mg
GlycoBlue™ Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant. The attached dye increases visibility of the pellet. This product is an ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution. Features of GlycoBlue™ Coprecipitant: • Ideal for RT-PCR • Increases pellet mass and visibility • Quantitative recovery of low concentrations (ng/mL) of nucleic acid • Prevents pellet loss in nuclease protection assays What is a coprecipitant? Coprecipitants are inert substances us...
Size: 5 x 300 µL
Manufactured by Sino Biological (China); Distributed by Life Technologies. Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications. This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus. N-terminal Sequence Analysis: Glu 99. Activity: 1. Mesured by emission analysis. 2. Measure excitation and emission spectra of GFP a...
Size: 5 x 5 µg