Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome bindi...
Size: 10 µg
Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Size: 0.5 mg
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Size: 25 mg
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Size: 5 ml
Immunogen: DNP Specificity: Specific for DNP (2,4-Dinitrophenol). Reactivity: DNP Applications: ELISA
Size: 100 µg
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg 2+ , dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5 kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.
Size: 100 reactions
Ubiquitin-Conjugating Enzyme E2W (Putative) (UBE2W), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human UBE2W (Q96B02-12) (Met 1-Cys 151) fused to a polyhistidine tag at the N-terminus. Activity: The activity of this protein has not been tested. Reconstitution: Dissolve the protein in sterile double distilled water to a...
Size: 50 µg
The 1 Kb DNA Extension Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 40 kb. The ladder consists of 8 fragments ranging in length from 1,018 to 8,144 bp in 1,018-bp increments, as well as bands of 506 bp, 517 bp, 1,636 bp, 10 kb, 15 kb, 20 kb, and 40 kb. For easy reference on agarose gels, the 5 kb band is two to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment DNA polymerase I (Klenow fragment).
Size: 100 µg
CAS-Block is a powerful universal blocking agent for immunochemistry methods including immunoperoxidase, HistoGold, Western blotting, etc. It has proven superior to traditional blocking methods and eliminates the need for species-specific serum blockers.
Size: 100 ml
This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Size: 400 reactions
POP-6™ is a DNA sequencing and fragment analysis reagent and consumable. It is a separation matrix for performing fragment analysis and sequencing on the 3100 Genetic Analyzer and 3100-Avant™ Genetic Analyzer. The POP-6™ Polymer is ideally suited for standard and rapid sequencing. Many Choices For the ABI PRISM® 310 and 3100 Genetic Analyzers, POP-4® polymers are designed for microsatellite and other genotyping applications, while POP-6™ polymers are the choice for standard and rapid sequencing. For the ABI PRISM® 3700 DNA Analyzer, POP-5™ polymer is the separation matrix of choice for high-th...
Size: 7000 µl
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Size: 1 instrument
PCR8⁄GW⁄TOPO TA KIT
Size: 1 each
The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscr...
Size: 20 reactions
Ambion® Silencer ® SelectGAPDH Positive Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs? Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algo...
Size: 250 nmol
The pShooter™ vectors are designed to localize recombinant proteins to the nucleus, mitochondria, endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter™ vector contains: Strong mammalian promoter (CMV or EF-1É) for constitutive expression of your protein of interest C-terminal c- myc epitope for detection with an Anti- myc Antibody Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability f1 origin for single-stranded rescue of sense DNA Neomycin resistance gene for selection of mammalian cells with Geneticin¤ selection agent Ampicillin resistance...
Size: 20 µg
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Size: 50 reactions
mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo , because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m 7 G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule. How mMESSAGE mMACHINE® kits work mMESSAGE mMACHINE® kits have a simplified reaction for...
Size: 25 rxns w⁄ manual
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Size: 300 units
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Size: 400 µL
The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Size: 10 µg
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Endogenous Control 96-well Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. The plate contains 16 commonly used housekeeping genes. The panel also contains 16 mouse orthologs of hum...
Size: 1 fast plate
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
The TaqMan® Fast Reagents Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5’ nuclease assay, in fast thermal cycling mode. The Eukaryotic 18S rRNA TaqMan® Assay consists of a 20X mix of two unlabeled PCR primers and a FAM™ dye labeled 18S TaqMan® MGB probe. Used With: 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module, 7900HT Fast Real-Time Service Upgrade, 7900HT System Fast 96-Well Block Upgrade Kit. For Research Use Only. Not for use in diagnostics procedures.
Size: 125 reactions
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Size: 4 tubes
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Size: 5 mg
Silencer ® Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided. • Premium-quality siRNA, highly purified and ready to use • Functionally tested in several common cell lines • Include Silencer ® Select modifications for enhanced specificity • For use in human, mouse, and rat cells What are Silencer ® Select siRNAs Silencer ® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, poten...
Size: 5 nmol
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Size: 5 nmol
Ambion® Silencer ® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using oth...
Size: 5 nmol
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Size: 10 x 500 ml
The CD54 (ICAM-1), clone YN1/1.7.4, APC (allophycocyanin) conjugate is a rat monoclonal antibody that detects mouse CD54 (ICAM-1). APC is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of APC in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: CD54 (ICAM-1), rat IgG2bκ monoclonal Label: APC (allophycocyanin) dye Validated Application:...
Size: 0.1 mg
This Goat Anti-Rabbit IgG Fc Antibody, Alkaline Phosphatase (AP) Conjugate is prepared from antibodies that have been adsorbed against bovine, horse and human serum proteins to minimize cross reactivity. Goat Anti-Rabbit IgG Fc Antibody, Alkaline Phosphatase (AP) Conjugate should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF). Applications: Goat Anti-Rabbit IgG Fc Antibody, Alkaline Phosphata...
Size: 1 mg
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS No preservative
Size: 1 ml
Protein A from Staphylococcus aureus, Cowan I Strain conjugated with highly purified horseradish peroxidase (RZ > 3.0). The product is liquid containing 10 mM phosphate, 0.15 M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol, and 0.1% proclin as preservative.
Size: 3 mg
Immunogen: Rat IgG (H+L) Specificity: Rat IgG (H+L) Applications: ELISA, Immunohistochemistry (FFPE), Immunofluorescence, Immunoprecipitation, WB
Size: 1 ml
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit: • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in less than 30 minutes Usi...
Size: 20 rxns w⁄ manual
RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving. Quality control RT-PCR Grade Water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL tubes. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously ...
Size: 10 × 1.5 ml
5% Digitonin is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Size: 1 ml
xMAP® Sheath Fluid (20X Concentrate) gives you the power to store more in less space and enhance your inventory control. xMAP® Sheath Fluid (20X Concentrate) lets you make 20 L of 1X Luminex® Sheath Fluid for use as the delivery medium of the sample to the optics component of the Luminex® 100/200™ system and FLEXMAP 3D® system: xMAP® Sheath Fluid (20X Concentrate) contains: Sheath Concentrate (1 L) Preservative Concentrate This material is also available from Luminex (Cat. No. 40-75680). For research use only. Not intended for human or animal therapeutic or diagnostic use.
Size: 1 L
Geltrex® hESC-qualified Ready-To-Use Reduced Growth Factor Basement Membrane Matrix is a pre-diluted matrix that can be taken directly from the refrigerator and dispensed into cell culture plates without further handling. Geltrex® hESC-qualified Ready-To-Use basement membrane matrix is optimized for stem cell culture applications using StemPro® hESC SFM or Essential 8™ media systems. Every lot is also functionally qualified using human embryonic stem cells to help reduce the need for lot screening. Geltrex® hESC-qualified Ready-To-Use matrix is: • Consistent—pre-dilution at a concentration of ...
Size: 50 mL
Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Using Nuclease-free Water Because this water is not DEPC-treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g., oocyte microinjection).
Size: 1 x 500 ml
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence Provides a stable baseline for multiplex quantitative PCR and RT-PCR Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
Size: 500 µl
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Size: 50 ml
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na 2 HPO 4 , and 2 mM KH 2 PO 4 . Each 10X PBS solution is ready to use upon dilution to the desired concentration. This molecular biology-grade PBS is certified RNase-free; it is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Size: 500 ml
The new Gibco® bottle has its own bottle weight for keeping order in the water bath! The bottle weight fits the new 500 mL and 1 L Gibco® bottles, keeping them upright in the water to further reduce your risk of contamination. Product Use For Research Use Only. Not for use in diagnostic procedures.
Size: 1 each
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Size: 500 ml
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and dea...
Size: 100 mL
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP manufacturing and quality system ACK Lysing Buffer is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Size: 100 mL
The Ion PI™ Chip Kit v2 contains chips for sequencing runs using the Ion Proton™ System, the first benchtop next-generation sequencer to offer fast, affordable human exome and transcriptome sequencing. The Ion PI™ Chip detects polymerase-driven base incorporation and translates this information into digital form. By eliminating the use of light-producing chemistry and optical systems, this revolutionary advance in next-generation sequencing technology makes massively parallel sequencing affordable to nearly every lab. The Ion PI™ Chip v2 provides: • 10 Gb of sequence data with a rapid run time...
Size: 8 chips
Angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human angiotensinogen (NP_000020.1) (Met 1-Ala 485) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween...
Size: 5 x 50 µg