The resDNASEQ® MDCK Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. T...
Size: 100 reactions
Retinoid-related orphan receptor gamma, ligand binding domain (RORG-LBD) is a 58.60 kDa recombinant human protein (amino acids 250-518) expressed as a GST-tagged fusion protein in insect cells. RORG-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.
Size: 50 μg
The Applied Biosystems® QuantStudio™ 3D Digital Chip Kit contains 96 digital PCR chips (eight 12-packs) and 1.5 mL of QuantStudio™ Digital PCR Master Mix (sufficient for 96 chips). The QuantStudio™ 3D Digital PCR Chip is at the heart of the QuantStudio™ 3D Digital PCR System. Manufactured from a silicon substrate and etched with 20,000 consistently-sized nanoscale reaction wells, the chip enables precise sample loading with an uncomplicated workflow. Each chip accomodates a single sample. QuantStudio™ 3D Digital PCR Master Mix is optimized for use with isolated DNA or cDNA, TaqMan® assays, and...
Size: 1 kit
The Illumina® TotalPrep™ RNA Amplification Kit is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Significantly improved sensitivit...
Size: 24 reactions
The Ion PGM™ Controls Kit v2 contains control genomic DNA and sequencing controls for 3 reactions and control libraries for 200 and 400 bp run configurations using the Ion Personal Genome Machine® (PGM™) semiconductor-based sequencing platform. The kit provides for greater experimental control through the use of quality control reagents for each step of the semiconductor-based sequencing workflow. The kit's quality control reagents allow you to assess sample quality, save time and reagents, and maximize productivity. The Ion PGM™ Controls Kit v2 provides you with: • Quality control tools for g...
Size: 1 kit
Ambion® RNaseAlert® QC System is a patent-pending technology detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Contain sufficient reagents for 5 × 96 high throughput assays. • Detects as little as 3.5 x 10 -7 units (~0.5 pg) of RNase A with a fluorometer • Simple, straightforward 30 min assay • Monitor RNase activity in column fractions during protein purification • Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluoresc...
Size: 480 assays
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
The Gateway® Vector Conversion System is designed to convert any vector into a Gateway® destination vector using restriction endonucleases and ligase. A Gateway® cassette containing att R recombination sites flanking a ccd B gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccd B gene. The Gateway® Vector Conversion System offers: • Conversion of any expression vector into a Gateway® destination vector • Three cassettes to construct a destinat...
Size: 1 kit
This pcDNA™3.1/Zeo (-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a reverse-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or ...
Size: 20 µg
The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Size: 1 kit
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR). • Pre-designed primers and TaqMan® probe eliminate assay design. • Rapid assay development guidelines can minimize optimization time. • Separate components provide flexibility in assay set-up. Comprehensive Guidelines Included Life Technologies® has developed a comprehensive set of ...
Size: 1 kit
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Size: 50 purifications
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Size: 10 × 50 µg
This is a purified rabbit anti-human DOPA Decarboxylase Monoclonal Antibody, Recombinant, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This DOPA Decarboxylase Monoclonal Antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human DOPA Decarboxylase . Antibody characteristics include: Applications: This DOPA Decarboxylase Monoclonal Antibody, Recombinant, has been validated for use in Western blot, ELISA, and immunohistochemistry Host species and isotype: This is a rabbit Monoclonal IgG against human DOPA...
Size: 100 µg
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Size: 1 standard plate
MitoTracker® Red FM is a far red-fluorescent dye (abs/em ~581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential.
Size: 20 × 50 µg
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 × 50 µl
The MagicMark™ XP Western Protein Standard is specifically designed for easy and convenient protein molecular weight estimation directly on Western blots. The standard consists of 9 recombinant proteins, each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the Western blot. The MagicMark™ XP Standard is compatible with all Western kits and substrates (chemiluminescent, chromogenic, and fluorescent) and offers: • Direct visualization of molecular weight ...
Size: 250 µL
One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. One Shot® TOP10 cells: • Maximize cloning efficiency in a single-tube format • Provide enhanced genomic DNA cloning capabilities Easy-to-use One Shot® format The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in th...
Size: 10 reactions
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Size: 1 kit
The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.
Size: 1 kit
Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L) Antibody is labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. The donkey anti-mouse IgG antibodies show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Size: 0.5 mL
The Alexa Fluor® 568 goat anti-mouse IgG 1 is labeled with our bright, red-fluorescent Alexa Fluor® 568 dye (which is spectrally similar to Rhodamine Red™ dye) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Size: 250 µl
The Alexa Fluor 633 goat anti—rabbit IgG is labeled with our far-red—fluorescent Alexa Fluor 633 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize crossreactivity, it has been adsorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum. We also provide a highly cross-adsorbed version of this same antibody (Catalog number: A-21071).
Size: 0.5 mL
Novex® 8% Tris-Glycine Midi Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Each box contains 10 gels. Features of these gels: • Individually packaged gels for optimal experiment design • Compatible with most protein standards • Usable for native and denaturing protein assays Choose the right gel format for your experiments Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–...
Size: 1 box
Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Size: 500 ml
5´-CCC↓GGG-3´ 3´-GGG↑CCC-5´ Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns. Concentration 10 U/ µL Contents Sma I 10X Buffer T (1mL) 10X Loading Buffer (1mL) Reaction Temperature 30°C Reaction Mixture Sma I 1 µL 10X Buffer T 2 µL 0.10% BSA 2 µL Substrate DNA Heat inactivation Enzyme inactivated by heating at 65°C for 20 minutes. S...
Size: 1000 units
The PureLink® Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ~30 minutes using PureLink® silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum. Advantages of using PureLink® Quick Gel Extraction Kit: • Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points • Complete the procedure in ~30 minutes ...
Size: 50 preps
Neutropilin-1 (NRP1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the human NRP1 isoform b (NP_001019799.1) (Met 1-Lys 644) fused to the polyhistidine tag at the C-terminus. Activity: Measured by using the Octet RED System, the affinity constant (Kd) of NRP1-His bound t...
Size: 5 µg
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Size: 10 ml
Novex® Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins, in their denatured state, on Novex® Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used. Separate native or denatured proteins Novex®Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex® Tris-Glycine gels, use Novex® Tris-Glycine Native Sample Buffer a...
Size: 5 L
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Size: 1 g
The 10 mM dNTP Mix consists of four nucleotides (dATP, dCTP, dGTP, dTTP) (2'-deoxynucleoside 5'-triphosphates), each at a concentration of 10 mM, in a solution of 0.6 mM Tris-HCl (pH 7.5). The 10 mM dNTP Mix is suitable for use in PCR, sequencing, fill-in, nick translation, cDNA synthesis, TdT-tailing reactions, and dilution of radiolabeled dNTPs.
Size: 100 µL
Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Size: 500 ml
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: • Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects • Easy optimization due to minimal cytotoxicity across a ...
Size: 0.1 ml
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g., in the presence of SDS or urea) • In the presence of metal chelating agents (e.g., EDTA) • At comparatively high temperatures (optimum digestion temperature is 65°C) Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization. Performance and quality testing E...
Size: 1.25 ml
Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Size: 10 reactions
NuPAGE® Transfer Buffer (20X) is a buffer used to transfer proteins from NuPAGE® Novex® gels to membranes for Western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. Related link Learn more about other Invitrogen™ products for your protein transfer needs .
Size: 125 ml
Same as VETEHDV/2 Single-well blocking ELISA kits enable sensitive and specific detection of pathogen-specific antibodies in animal samples, allowing for reliable and rapid screening and detection of seropositive animals. How the kit works: 1. Samples are added to the specific antigen-coated microplate. If present in the sample, specific anti-pathogen antibodies bind to the antigen and form antigen-antibody complexes. 2 . After washing, an anti-pathogen peroxidase-labeled conjugate is added, which binds to any remaining unbound antigen. 3 . After a second washing, a chromogenic substrate is ad...
Size: 192 tests
The Diagenode™ Bioruptor® Pico Ultrasonicator is an all-in-one shearing system optimized for shearing volumes as small as 5 µL up to 2 mL. This wide range enables a variety of applications such as DNA shearing for next-generation-sequencing library preparation, RNA shearing, and chromatin shearing. Shearing is a critical step in next-generation-sequencing library preparation, chromatin immunoprecipitation, and ChIP-seq. Key Product Features: • All-in-one solution with advanced timing control and regulated cooling • Versatile—shears sample sizes from 5 µL–2 mL • Wide range of applications: next...
Size: 1 unit
TC 100 Insect Medium was originally designed for the growth of insect cells, including Spodeptera frugiperda (Sf9), and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). Life Technologies offers a variety of Gibco® Media options for a range of insect cell culture applications . This IPL-41 Insect Medium is modified as follows: With • L-glutamine • Tryptose phosphate broth • Sodium bicarbonate
Size: 500 ml
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Size: 10 packs, 2,000 reactions
This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Size: 1000 reactions
Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Size: 25 ml
Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase ...
Size: 1 ml
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Size: 250 reactions
PathAmp™ FluA Reagents are a set of highly specific, universal influenza primers and a high-fidelity master mix for the amplification of all eight influenza A genomic segments in a single tube. The reaction produces cDNA fragments that range in size from 900 bp to 2.4 kb, which can be used as an input into the Ion PGM™ library preparation workflow. The PathAmp™ FluA Reagents provide a rapid, accurate, and sensitive solution for influenza A viral sequencing on the Ion PGM™ Sequencer. The resulting data can be used for future outbreak monitoring as well as routine surveillance. Benefits of the P...
Size: 50 reactions
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
Size: 20 reactions