The Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation. How it works The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate. Calcium phosphate facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis. The method was first developed by Graham and van der Ebb and was later modified by Wigler. The procedure is routinely used to transfect a wide variety of cell types f...
Size: 75 reactions
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Size: 1 kit
The Neon® Transfection System 100 µL Kit is designed specifically for use with the Neon® Transfection System . Use this kit for transfection volumes of 100 µL, containing 5 × 10 5 –2 × 10 6 adherent cells or 1 × 10 6 –5 × 10 6 suspension cells. Cells that have been transfected using the included 100 µL Neon® Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
Size: 25 × 2 reactions
The Neon® Transfection System 10 µL Kit is designed specifically for use with the Neon® Transfection System . Use this kit for transfection volumes of 10 µL, containing 5 × 10 4 –2 × 10 5 adherent cells or 1 × 10 5 –5 × 10 5 suspension cells. Cells that have been transfected using the included 10 µL Neon® Tips are ready to be washed and plated into a multi-well culture dish.
Size: 96 × 2 reactions