Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Alexa Fluor® 594-5-dUTP (590/615 nm) • L...
Size: 25 µl
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Size: 100 µl
Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation. Performance and ...
Size: 50 nmol
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm) • Length...
Size: 25 µl
The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.
Size: 100 µl
Ambion® Cap Analog [m 7 G(5')ppp(5')G] is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. These molecules are translated more efficiently in in vitro translation systems than uncapped mRNAs. 25 A 254 U supplied. Substitution of cap analog for a portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a corresponding fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. It is important that in vitro transc...
Size: 1 tube
The 100 mM dNTP Set consists of four deoxynucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. The deoxynucleotides are suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT tailing reactions. The nucleotides are supplied as ready-to-use solutions.
Size: 4 x 250 µL
Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365. Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapt...
Size: 5 mg
The 10 mM dNTP Mix consists of four nucleotides (dATP, dCTP, dGTP, dTTP) (2'-deoxynucleoside 5'-triphosphates), each at a concentration of 10 mM, in a solution of 0.6 mM Tris-HCl (pH 7.5). The 10 mM dNTP Mix is suitable for use in PCR, sequencing, fill-in, nick translation, cDNA synthesis, TdT-tailing reactions, and dilution of radiolabeled dNTPs.
Size: 100 µL
ATP is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's ATP is 10mM in water.
Size: 500 µl
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
Size: 100 mg
GeneAmp® dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. GeneAmp® dNTPs with dUTP can be used to generate dU-containing PCR products in a carry-over prevention strategy in conjunction with AmpErase ® Uracil N-glycosylase (UNG). dNTPs have high purity, making them ideal for use in PCR reactions or other applications. dNTPs with dUTP are optimized for use in carry-over prevention. Guaranteed for PCR performance when used with GeneAmp® PCR core reagents and GeneAmp® instrument systems. Premixed dNTP blends are available in various conce...
Size: 12 x 1 ml
Invitrogen offers primers for PCR amplification that complement many of the vectors currently available. All primers are: Desalted and purified by gel filtration Assayed for function by PCR amplification Provided in 2 µg quantity T7: 5´- TAATACGACTCACTATAGGG- 3´
Size: 2 µg
Random Decamers are the same primers currently included in the RETROscript® Kit (Cat. No. AM1710). They are provided at a stock concentration of 50 µM, and are functionally tested using the RETROscript® Kit. Using random primers Generally, reverse transcription reactions are primed with one of the following types of primers: random sequence oligonucleotides, oligo(dT), or an oligonucleotide that can hybridize with the specific RNA under study. The type of first-strand primer used for reverse transcription is mostly based on user preference. For cDNA library construction or cDNA labeling applic...
Size: 80 µl
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Size: 80 µl
For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Size: 1 set