The resDNASEQ® NS0 Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high m...
Size: 100 reactions
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Size: 1 kit
The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Size: 1 kit
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Size: 25,000 reactions
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is i...
Size: 1,000 reactions
The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli . The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor and time saving —no need for extra steps to remove endotoxin and other contaminants • High-purity, low-endotoxin product —pure enough for mammalian cell transfection • Versatility —purify any type and size of plasm...
Size: 25 preps
Th e GeneBLAzer® Jurkat Control Kit contains constitutively expressing (CMV- bla ) and wildtype Jurkat cells to provide positive and negative controls, respectively, for evaluating beta-lactamase activity (Figure 1).
Size: 1 kit
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
The MEGAshortscript™ T7 Kit is designed for high yields of in vitro - transcribed RNA products, in the 20–500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maxi...
Size: 25 rxns w⁄ manual
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides: • Rapid, non-hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and convenient column purification The PureLink® RNA Mini Kit provides rapid purification of total RNA from a...
Size: 50 preps
The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA. A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Size: 200 reactions
The ApoTarget™ APO-BRDU Kit is a two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
Size: 60 tests
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Size: 96 preps
The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase. Using the MEGAscript® T7 Kit The MEGAscript® T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the...
Size: 25 reactions
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Size: 1 kit
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Size: 1 kit
Perform up to 2,000 PCR reactions with Applied Biosystems® TaqMan® PCR Core Reagent kit, 10-Pack. Applied Biosystems® TaqMan® PCR Core Reagents, 10-pack provides the individual components needed to perform up to 2,000 PCR reactions (50 µl each). Features of the TaqMan® PCR Core Reagent Kit: • Separate components, packaged in 10 tubes, provide maximum flexibility in assay setup • AmpliTaq Gold® DNA polymerase, a chemically modified form of AmpliTaq® DNA Polymerase, improves specificity and yield • AmpErase® UNG protects against subsequent re-amplifications from PCR products containing dU to min...
Size: 10 packs, 2,000 reactions
The MagMAX™-96 for Microarrays Total RNA Isolation Kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates. The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis. The kit includes sufficient reagents for 96 reactions. Advantages of the MagMAX™-96 for Microarrays Total RNA Isolation Kit: • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent® with a superior...
Size: 96 purifications
The LIVE/DEAD® Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer. • Stable—dyes are freeze dried in separate vials to maintain stability • Robust—staining pattern is the same before and after fixation • Bright signal—allows for easy distinction between live/dead cells in single channel Stable Unlike products that are so...
Size: 1 kit