Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 100 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, an...
Size: 100 mL
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Size: 6 µg
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016). The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Size: 10 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions