The LIVE/DEAD® Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer. • Stable—dyes are freeze dried in separate vials to maintain stability • Robust—staining pattern is the same before and after fixation • Bright signal—allows for easy distinction between live/dead cells in single channel Stable Unlike products that are so...
Size: 1 kit
The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced. Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of ...
Size: 3 x 10
ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 x 50 µL
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Size: 100 mL
The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Size: 10 x 500 mL
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Size: 500 mL
Applied Biosystems® GeneAmp® Thin-Walled Reaction Tubes enable fast heat transfer for precise PCR in 0.5 mL PCR Systems. • Thin plastic enables fast heating and cooling for accurate results • Flat, frosted caps for easy marking • Captive lid with positive-click closure assures proper seating • Polished surface and conical bottom allow maximum sample recovery • Autoclaved tubes provide a clean, controlled start Rapid Heat Transfer The GeneAmp® Reaction Tube is made of specially engineered polypropylene and is optimized for use with the Veriti™ 60-Well Thermal Cycler and the Dual 96-Well GeneAmp...
Size: 1000 tubes
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex® xMAP® Technology. These assays make use of Luminex® MagPlex™, or magnetic microspheres, and the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments. Note: It is recommended that the Magnetic 96-Well Separator be used with a standard 96-well flat-bottom plate such as Grein...
Size: 1 each
• The Heat Equalizing Block is an aluminum conduction plate for use with the SSP UniTray and other laboratory procedures that require even heat distribution during thermal cycling. The Heat Equalizing Block is set on top of the SSP UniTray while thermal cycling to ensure even heat distribution • The Membrane Comb Loading Tray is for convenient benchtop loading of 64-lane membrane combs. Polycarbonate tray with two rows of 64 wells, each 2 mm wide • HLA Beyond Tears - By Glenn E. Rodey, Second Edition (De Novo, Inc., Durango, CO 2000) Serves as an introductory text for individuals who are inter...
Size: 1 each
The Applied Biosystems® MicroAmp® Fast Optical 96-Well Reaction Plate is an integral component of the Applied Biosystems® Fast PCR Systems, which reduces PCR reaction time from 2 hours to as little as 25 minutes. • Maximum thermal conductivity for precise thermal cycling • Reproducible, specific, and sensitive PCR results in just 25 minutes • Validated with other system components for consistent results Increased Thermal Contact for Faster, More Uniform Heating The advanced design of the MicroAmp™ Fast 96-Well Reaction Plate dramatically increases its thermal contact with the Applied Biosystem...
Size: 10 plates
The ViiA™ 7 Real-Time PCR System combines all of the qPCR features you want in a single high performance instrument, so that you can optimize your research productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, the ViiA™ 7 System offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high-productivity real-time PCR accessible to any size l...
Size: 1 instrument
The Applied Biosystems® MicroAmp® Optical 384-Well Reaction Plate with Barcode is designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 384-well format, is designed to work with Applied Biosystems® 384-well Real-Time PCR systems and thermal cyclers. • Frosted to minimize interfering fluorescence from wells of cycling block • Screened to eliminate auto-fluorescent plates • Unique design provides a barrier to ambient air to help ensure well-to-well temperature uniformity • E...
Size: 500 plates
The Blotting Roller is an 8.6 cm wide roller designed for easy and efficient removal of bubbles when assembling gel/membrane sandwiches for blotting both E-PAGE™ Gels and mini-gels such as NuPAGE® and Novex¤ Tris-Glycine Gels. It consists of a Delrin roller attached to a stainless steel handle. The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.
Size: 1 unit
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Size: 1 x 10 L
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Opti-MEM® I modifications for a range of cell culture applications. This Opti-ME...
Size: 500 mL
The MicroAmp® Cap Installing Tool helps you install strips of MicroAmp® caps onto MicroAmp® reaction tubes in a tray⁄retainer set or 96-well reaction plate. • Available in handle style • Seal 8 or 12 cap strips • Recessed cup for pressing caps onto tubes • Convenient tool ensures proper sealing Press and Seal Caps The MicroAmp® Cap Installing Tool enables you to ergonomically seal MicroAmp® reaction tubes and plates. This tight seal will prevent well-to-well contamination and sample loss. Used With MicroAmp® Optical 8-Cap Strip, MicroAmp® Optical 8-Tube Strip, 0.2 ml, MicroAmp® Optical 96-Well...
Size: 1 tool
Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion. • Gibco® microbiological media • Convenient ready-to-use liquid format • 1 L size available YPD Broth Recipe YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris ( P. pastoris ) and Saccharomyces cerevisae ( S. cerevisae ) yeast strains. Formulation per One Liter: 10 g Yeast extract 20 g Bac...
Size: 1 L
Custom Media: EHAA Medium Click's, 500 mL
Size: 500 mL
The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Size: 1 each