This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo , because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m 7 G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule. How mMESSAGE mMACHINE® kits work mMESSAGE mMACHINE® kits have a simplified reaction for...
Size: 25 reactions w⁄ manual
NucRed® Live 647 ReadyProbes® Reagent is a cell-permeant nuclear stain that emits bright, far-red fluorescence when bound to DNA. Like other ReadyProbes® reagents, NucRed® Live 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. NucRed® Live 647 ReadyProbes® Reagent provides: • Exceptionally bright far-red fluorescence well separated from blue, green, and red fluorophores • Rapid staining of live or fixed cells without wash steps • Ready-to-use liquid formulation in convenient dropper ...
Size: 1 kit
Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes® Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of Propidium Iodide ReadyProbes® Reagent: • Ready-to-use liquid propidium iodide formulation • Rapid staining of dead cells without wash steps • Ready-to-use liquid formulation in convenien...
Size: 1 kit
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Size: 500 g
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Size: 100 mL
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Size: 1 kit
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions
The highly deionized (Hi-Di) Formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems. The smaller configuration (5 ml) minimizes the number of freeze⁄thaw cycles compared to the larger 25ml bottle and may be a better fit when running a lower number of samples. For Research Use Only. Not for use in diagnostics procedures.
Size: 4 x 5 mL
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Size: 6 µg
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Size: 500 mL
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Size: 80 µL
Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Size: 100 µg
Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Size: 100 mL
These individual EcoRI & MseI Adaptors can be used in the Restriction Ligation reaction. For Research Use Only. Not for use in diagnostics procedures.
Size: 100 reactions
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 100 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, an...
Size: 100 mL
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016). The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Size: 10 µg
Ephrin A5 (EFNA5), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mature form (Met 1-Asn 203) of mouse EFNA5 long isoform (O08543-1) fused to a polyhistidine tag at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EPHA...
Size: 5 µg