Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Size: 5 ml
The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superi...
Size: 1 kit
The CellSensor® NFκB-bla RAW264.7 mouse macrophage cell line contains a beta-lactamase reporter gene under control of a stably integrated NFκB response element. This cell line is a clonal population isolated in response to lipopolysaccharide by flow cytometry. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and has been validated for Zfi-factor and EC50 concentrations of the TLR7 ligand imiquimod. Additional data using alternate stimuli are shown. The molecules responsible f...
Size: 1 vial
Neurotrophins (NGF, BDNF, NT-3, and NT-4) and their transmembrane receptors (TrkA, TrkB, TrkC, and P75NTR) play important roles in the regulation of neuronal and non-neuronal cell proliferation, differentiation, survival, and death. Neurotrophin signaling also mediates higher-order neuronal activities, such as learning, memory, and behavior. Alterations in neurotrophin levels and their receptors have been implicated in neurodegenerative disorders, such as Alzheimer's disease and Huntington's disease, as well as psychiatric disorders. Neurotrophins propagate their signal through activating mult...
Size: 1 vial
The CellSensor™Myc- bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc . This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi aga...
Size: 1 vial
Jak/Stat signaling pathways play essential roles in the cellular responses to distinct cytokines. One of the Jak/Stat pathways, Jak2/Stat5, mediates cell proliferation in response to Interleukin - 3 (IL - 3), prolactin, erythropoietin (Epo), and granulocyte - macrophage colony stimulating factor (GM - CSF). JAK2 gene knockout causes embryonic lethality due to defective erythropoiesis, suggesting that the Jak2/Stat5 pathway plays an important role in red blood cell formation. The recent discovery of an activating mutation in JAK2 (V617F) present in a high percentage of myeloproliferative diseas...
Size: 1 vial
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency® Stbl2™ Competent Cells offer: • >1 x 10 9 transformants/µg plasmid DNA for cloning rare sequences • Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A—allows cloning of methylated genomic sequences • A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV ...
Size: 10 reactions
For Academic pricing please login or contact us at firstname.lastname@example.org. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Size: 8 × 10
The CellSensor® Gli-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into NIH 3T3 cells. This cell line is a clonal population isolated by flow cytometry in response to the mouse sonic hedgehog (mShh) ligand. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, as well as validated for Z'-factor and EC50 concentrations of Shh. Additional data using known inhibitors or activators of the pathway have also been...
Size: 1 vial
LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The JAK2⁄STAT5 signaling pathway plays an essential role in blood cell formation in response to cytokines such as GM-CSF, IL-3, and EPO. The recent discovery of an activating mutation in JAK2 (V617F) present in high percentage of myeloproliferative disease (MPD) patients suggests that this mutant JAK2 activity is a potential therapeutic target for certain forms of MPD. The assay described in this summary makes use of a cell line engineered for ex...
Size: 1 vial
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄ lac Z. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
Size: 3 × 10
MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) Using MAX Eff...
Size: 5 × 100 µl
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Size: 5 bottle
alamarBlue® is a redox indicator that yields a colorimetric change and a fluorescent signal in a response to a metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies. Applications: • Cell proliferation determinations —alamarBlue® is water soluble, stable in culture and non-toxic • Cell viability assays, including cytotoxicity —metabolic activity and dye generation changes in proportion to altered viability and cell damage • Cytokine assays —measure cytokine-induc...
Size: 100 ml
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Size: 100 mL
One Shot® ccd B Survival™ 2 T1 R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/µg plasmid DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccd B Survival™ 2 T1 R E. coli strain offers the following features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fhu A phenotype) • ...
Size: 10 transformations
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Size: 100 mL
ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX™ DH10B™ cells: • Maximize yield of transformants from limited cloning products • Permit efficient cloning of methylated DNA • Are resistant to T1 and T5 bacteriophages • Support blue/white screening by α-complementation on plates containing ...
Size: 25 reactions
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Size: 1 kit