StemPro® Neural Stem Cells are cryopreserved human fetal brain-derived neural stem cells (NSCs). These cells are manufactured under Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility and are available for Research Use Only. They are isolated and expanded in culture to passage 4 under a low oxygen (ischemic tolerant), proprietary manufacturing process using FBS-supplemented media and then cryopreserved in an animal origin-free cryopreservation medium. The cells are supplied at a concentration of 5 x 10 6 viable cells/vial. A Certificate of Analysis is av...
Size: 5 x 10
T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄ lac Z. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
Size: 3 × 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Size: 1 ml
Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase ...
Size: 1 ml
StemPro® BM Mesenchymal Stem Cells are cryopreserved human bone marrow mesenchymal stem cells (BM MSCs). These Off-the-Shelf cells are manufactured to meet Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility and available for Research Use Only. They are isolated and expanded in culture to passage 4 under a low oxygen (ischemic tolerant (it)) proprietary clinical-scale manufacturing process using Bovine Growth Serum (BGS)-supplemented media and cryopreserved in an animal origin free cryopreservation medium. The cells are supplied at 1x10 6 and 5x10 6 viab...
Size: 5 x 10
StemPro® Neural Stem Cells are cryopreserved human fetal brain-derived neural stem cells (NSCs). These cells are manufactured under Good Manufacturing Practice (GMP) manufacturing standards in a California-licensed facility and are available for Research Use Only. They are isolated and expanded in culture to passage 4 under a low oxygen (ischemic tolerant, proprietary manufacturing process using FBS-supplemented media and then cryopreserved in an animal origin-free cryopreservation medium. The cells are supplied at a concentration of 1 x 10 6 viable cells/vial. A Certificate of Analysis is ava...
Size: 1 x 10
The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superi...
Size: 1 kit
Gibco® Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES®). The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage. S2 cells grow at room temperature in ambient CO 2 as a loose, semi-adherent monolayer in tissue culture flasks and in suspension in spinners and shake flasks. Drosophila S2 cells (frozen in Gibco® Schneider's Medium) feature: • Expression of protein transiently or through ...
Size: 1 ml
The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced. Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of ...
Size: 3 × 10
Cryopreserved Human Kupffer Cells are isolated from adult human livers. Each vial contains a minimum of one million viable cells. These easy-to-use cells provide a convenient way to produce hepatocyte and Kupffer cell co-cultures for the study of various hepatic functions.These cells typically have high viabilities and in vivo-like expression levels. • Viability routinely >90% • Purity routinely >90% • Respond to activation with lipopolysaccharides (LPS) • Minimum 1 million viable cells per vial • Protocols for hepatocyte co-culture available Kupffer Cells Provide for Improved Physiological Mo...
Size: 1 vial
Cryopreserved human hepatocytes for use in transporter-mediated uptake applications. Cells per vial: 4-8 million Prototypical hepatic uptake transporter substrates taurocholate and estradiol-17β-glucuronide have been assessed to qualify the transport function of hepatic NTCP (SLC10A1) and OATP1B14/3 (SLCO1B1/3), respectively. The transport of each substrate (radiolabeled) was monitored by scintillation analysis and active transport values (37°C - 4°C) determined by linear regression. Get the Results You Need with GIBCO® Hepatocytes High quality hepatocytes, with high viabilities, in vivo -like...
Size: 1.5 ml
Size: 1 plate