HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Size: 1 set
InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Size: 500 ml
Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Size: 1 L
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µl
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Size: 100 µl
MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
Size: 20 × 50 µg
LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Effective labeling of live cells at nanomolar concentrations • Live-cell imaging • With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy5 or MitoTracker® Deep Red Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This all...
Size: 5 × 50 µl
MitoTracker® Red FM is a far red-fluorescent dye (abs/em ~581/644 nm) that stains mitochondria in live cells and its accumulation is dependent upon membrane potential.
Size: 20 × 50 µg
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol).
Size: 25 mg
LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Good retention after aldehyde fixation • Effective labeling of live cells at nanomolar concentrations • Live cell imaging • Excitation/Emission: 577/590 nm Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate ...
Size: 20 × 50 µl
UltraPure™ Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. It is available as an aqueous solution containing 10 mg/ml UltraPure™ Ethidium Bromide and high-purity water. • Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels • Versatile: Detect DNA or RNA • Easy to Use: Can be used as-is or diluted to the desired concentration UltraPure™ Ethidium Bromide is an aromatic compound with a phenanthridine core (see structure below). Ethidium bromide has excitation maxima at 300 nm and 520 nm ...
Size: 10 ml
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Size: 1 each
The Bolt® Mini Blot Module Gasket is a replacement part for the Bolt® Mini Blot Module. The gasket fits on the cathode core (-) of the blot module. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core o...
Size: 1 unit
Replacement Parts for Electrophoresis Apparatus
Size: 1 each
DryEase® Mini-Gel Cellophane is one component of the DryEase® Mini-Gel Drying System , which is a simple, easy-to-use kit for drying gels evenly using passive evaporation. The kit allows gels to be assembled on a base between two open frames, so that both outer surfaces of the gels are exposed to air. Consequently, drying is even and cracking is minimized. The proprietary Gel-Dry™ Drying Solution modulates the rate of water release to ensure crack-free gels. With the DryEase® System, gels are dried overnight with uniform tension on all points of the gel to eliminate cracking. Applications: Gel...
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Size: 4 pads
The Blotting Roller is an 8.6 cm wide roller designed for easy and efficient removal of bubbles when assembling gel/membrane sandwiches for blotting both E-PAGE™ Gels and mini-gels such as NuPAGE® and Novex¤ Tris-Glycine Gels. It consists of a Delrin roller attached to a stainless steel handle. The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.
Size: 1 unit
The Blotting Tweezer is a replacement part for the Bolt™ Mini Blot Module. It is for general use of handling nitrocellulose or PVDF membranes. About the Bolt™ Mini Blot Module The Bolt™ Mini Blot Module is a wet transfer device used exclusively with the Bolt™ Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt™ Mini Blot Module is compatible with Bolt™ Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the...
Size: 1 unit