The PureLink® PCR Purification Kit provides rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products. The simple PureLink® bind/wash/elute procedure: • Provides two binding buffer options for fragments >100 bp or >300 bp • Can be performed in either single-column or 96-well plate format • Takes Using the PureLink® PCR Purification Kit Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use ...
Size: 50 purifications
The MICROB Express ™ Bacterial mRNA Enrichment Kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid purification of bacterial mRNA Isolating mRNA from bacteria has been virtually impossible, until now. The...
Size: 20 reactions
The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli . The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor and time saving —no need for extra steps to remove endotoxin and other contaminants • High-purity, low-endotoxin product —pure enough for mammalian cell transfection • Versatility —purify any type and size of plasm...
Size: 25 preps
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Size: 12 samples
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Size: 1 kit
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides: • Rapid, non-hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and convenient column purification The PureLink® RNA Mini Kit provides rapid purification of total RNA from a...
Size: 50 preps
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Maximize Total ...
Size: 96 preps
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Size: 1 kit
The PureLink® Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ~30 minutes using PureLink® silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum. Advantages of using PureLink® Quick Gel Extraction Kit: • Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points • Complete the procedure in ~30 minutes ...
Size: 50 preps
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Size: 4 preps
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Size: 5 × 96 preps
The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA + RNA isolation. The columns are sterilized and RNase-free.
Size: 200 columns
The MagMAX™-96 for Microarrays Total RNA Isolation Kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates. The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis. The kit includes sufficient reagents for 96 reactions. Advantages of the MagMAX™-96 for Microarrays Total RNA Isolation Kit: • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent® with a superior...
Size: 96 purifications
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit: • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in less than 30 minutes Usi...
Size: 20 rxns w⁄ manual
The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The PureLink® Viral Mini Kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and g...
Size: 50 preps
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Size: 50 purifications
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRIzol® LS Reagent: • Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein...
Size: 200 ml
Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes. • Processed faster than ultrafiltration columns • Rapid and efficient separations • Buffer is not preselected • Convenient 20-100 µL sample size • Dry gel format • Room Temperature Storage • Microcentrifuge format Designed for Per...
Size: 50 plates
DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Size: 100 mL