The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Size: 1 each
The Covaris® M220 miniTUBE Prep Station enables the easy loading of sample into clear, blue, or red miniTUBEs. A tilted stand is included that has been designed to help recover the entire sample from the miniTUBE. Each package contains 1 preparation station.
Size: 1 each
The Ion PI™ Template OT2 200 Kit v2 contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System for Ion Proton™ System semiconductor sequencing. As an integral component of the Ion Proton™ semiconductor sequencing workflow, the Ion PI™ Template OT2 200 Kit v2 is designed to make high-quality templated Ion PI™ Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion PI™ Template OT2 20...
Size: 8 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Size: 6 µg
The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product th...
Size: 50 reactions
The pFastBac™ Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016). The vector has two strong promoters, the polyhedrin promoter and the p10 promoter, for high-level expression. An expression control is included that expresses both CAT and β-glucuronidase (gus).
Size: 10 µg
The Covaris® M220 miniTUBE Accessory Kit is ideally suited for Ion PGM™ users requiring large DNA fragments for their applications (mate-paired libraries, etc), providing tight DNA fragment distributions centered on 2,000, 3,000 or 5,000 bp. The kit includes a holder, 25 clear miniTUBEs for 2 kb fragments, 25 blue miniTUBEs for 3 kb fragments, and 25 red miniTUBEs for 5 kb fragments. The kit also includes a preparation station for easy loading and unloading of sample into the miniTUBEs.
Size: 1 kit
The Covaris® M220 miniTUBE Holder is required to process the sample tube in the M220 Focused-ultrasonicator™. This holder is compatible with clear, blue, and red miniTUBEs. Each package contains 1 holder station.
Size: 1 kit
The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Size: 48 reactions