The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Size: 1 kit
DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Size: 100 mL
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRIzol® LS Reagent: • Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein...
Size: 200 ml
PathAmp™ FluA Reagents are a set of highly specific, universal influenza primers and a high-fidelity master mix for the amplification of all eight influenza A genomic segments in a single tube. The reaction produces cDNA fragments that range in size from 900 bp to 2.4 kb, which can be used as an input into the Ion PGM™ library preparation workflow. The PathAmp™ FluA Reagents provide a rapid, accurate, and sensitive solution for influenza A viral sequencing on the Ion PGM™ Sequencer. The resulting data can be used for future outbreak monitoring as well as routine surveillance. Benefits of the P...
Size: 50 reactions
The PureLink® PCR Purification Kit provides rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products. The simple PureLink® bind/wash/elute procedure: • Provides two binding buffer options for fragments >100 bp or >300 bp • Can be performed in either single-column or 96-well plate format • Takes Using the PureLink® PCR Purification Kit Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use ...
Size: 50 purifications
RNase AWAY ® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY ® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and quality testing RNase AWAY ® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Size: 250 ml
Ambion® RNA secure ™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency ...
Size: 10 ml
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Size: 1 kit
NucGreen™ Dead 488 ReadyProbes™ Reagent is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucGreen™ Dead 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and dr...
Size: 1 kit
NucRed™ Live 647 ReadyProbes™ Reagent is a cell-permeant nuclear stain that emits bright, far-red fluorescence when bound to DNA. Like other ReadyProbes™ reagents, NucRed™ Live 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. NucRed™ Live 647 ReadyProbes™ Reagent provides: • Exceptionally bright far-red fluorescence well separated from blue, green, and red fluorophores • Rapid staining of live or fixed cells without wash steps • Ready-to-use liquid formulation in convenient dropper ...
Size: 1 kit
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Size: 1 ml
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Size: 1 kit
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Size: 1 ml
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes™ Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Live ReadyProbes™ Reagent: Ready-to-use liquid formulation of high-purity Hoechst 33342 No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or cell cult...
Size: 1 kit
NucRed™ Dead 647 ReadyProbes™ Reagent is a cell impermeant stain that emits bright far-red fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live cell assays. NucRed™ Dead 647 reagent is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucRed™ Dead 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip a...
Size: 1 kit
Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes™ Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of Propidium Iodide ReadyProbes™ Reagent: • Ready-to-use liquid propidium iodide formulation • Rapid staining of dead cells without wash steps • Ready-to-use liquid formulation in convenien...
Size: 1 kit
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Size: 50 purifications
The MICROB Express ™ Bacterial mRNA Enrichment Kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid purification of bacterial mRNA Isolating mRNA from bacteria has been virtually impossible, until now. The...
Size: 20 reactions
The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli . The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor and time saving —no need for extra steps to remove endotoxin and other contaminants • High-purity, low-endotoxin product —pure enough for mammalian cell transfection • Versatility —purify any type and size of plasm...
Size: 25 preps
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps