T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Size: 2 x 2,500 units
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinu...
Size: 100 reactions
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Size: 2 × 1 ml
This high-purity Ambion® Taq Polymerase is isolated from an E. coli strain that over-expresses this gene. Its heat stability allows DNA polymerization in the 5' to 3' direction during repeated heat denaturation and reannealing steps in the presence of dNTPs, a DNA template, and complementary primers. It is supplied in one tube containing 250 U at a concentration of 5 U/ µL. It can be used in PCR and RT-PCR reactions. Unit Definition: One unit incorporates 10 nmol of deoxynucleotides into acid insoluble material in 30 min at 74°C. SuperTaq ™ is a trademark of, and is manufactured by Enzyme Tech...
Size: 250 units
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme: • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy number amplifications • Successful multiplex PCR saves time and reagents • Includes GeneAmp® 10X PCR Buffer II with MgCl 2 Hot-start activation The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resultin...
Size: 250 units
Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. Features of Platinum® Taq DNA Polymerase, High Fidelity: • Fidelity —greater than six times higher fidelity than Taq DNA polymerase • Amplicon size —amplification of fragments up to 15 kb (see ...
Size: 5,000 reactions
Tfi DNA Polymerase is a new non- Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq , Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a co...
Size: 5,000 reactions
AccuPrime™ Taq DNA Polymerase, High Fidelity, amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase, High Fidelity, you'll get: • The highest specificity and yield for the most robust PCR amplification • 9-fold higher fidelity than Taq DNA polymerase alone • Mimimal optimization steps, even with non-optimized primer sets • Efficient amplification of targets over a broad...
Size: 1,000 reactions