Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Size: 2 × 1 ml
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinu...
Size: 100 reactions
Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. Features of Platinum® Taq DNA Polymerase, High Fidelity: • Fidelity —greater than six times higher fidelity than Taq DNA polymerase • Amplicon size —amplification of fragments up to 15 kb (see ...
Size: 5,000 reactions
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme: • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy number amplifications • Successful multiplex PCR saves time and reagents • Includes GeneAmp® 10X PCR Buffer II with MgCl 2 Hot-start activation The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resultin...
Size: 250 units
This kit contains components needed to perform up to 1000 assays using TaqMan® probes. The kit employs AmpliTaq Gold® DNA polymerase for enhanced performance, 10X TaqMan Buffer A, and magnesium chloride. The components are provided in bulk and enable flexibility in assay setup. Separate Components Provide Flexibility in Assay Set-Up AmpliTaq Gold® DNA polymerase increses yield and gives a more robust 5' nuclease assay than AmpliTaq® DNA polymerase. Passive Reference I in the 10X TaqMan® Buffer A normalizes signal in all TaqMan® Assay reactions and corrects inter-well signal variation caused by...
Size: 1000 reactions
RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving. Quality control RT-PCR Grade Water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL tubes. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously ...
Size: 10 × 1.5 ml